(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study
文献类型：期刊论文
| 作者 | Fu, Yunbin1,4; Wang, Mengyu4; Wang, Wenxia4; Tuo, Yaqin5; Guo, Zhimou3; Du, Yuguang2; Yin, Heng4 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 81页码:1069-1073 |
| 关键词 | (1 -> 3)-beta-d-glucan Oligosaccharides Monomer Degree Of Polymerization Hydrogen Peroxide Tobacco |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2015.09.028 |
| 文献子类 | Article |
| 英文摘要 | In order to produce highly purified (1 -> 3)-beta-D-glucan oligosaccharides ((1 -> 3)-beta-D-GOS) monomers, a hydrophilic interaction liquid chromatography (HILIC) system with X-Amide stationary phase was performed. Nine (1 -> 3)-beta-D-GOS monomers with degree of polymerization (DP) from 2 to 10 were successfully separated. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) demonstrated that these monomers were with high purity. Furthermore, a hydrogen peroxide (H2O2) online detection method was established to monitor H2O2 releases in tobacco cells. This is the first report on nine consecutive (1 -> 3)-beta-D-GOS monomers purification and its effect upon H2O2-releasing in plants. Itwas found that (1 -> 3)-beta-D-GOS monomers with higher DP induced stronger defense responses in plants, which will pave the way for elucidating the relationship between (1 -> 3)-beta-D-GOS and biological activities. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | TOBACCO SUSPENSION CELLS ; BETA-1,3 GLUCAN SULFATE ; DEFENSE RESPONSES ; PLASMOPARA-VITICOLA ; RESISTANCE ; GRAPEVINE ; DEATH ; OLIGOGALACTURONIDES ; CHROMATOGRAPHY ; SEPARATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000367408300133 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171518]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Du, Yuguang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Separat Mat & Separat Method Grp, Dalian 116023, Liaoning, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Grp, Dalian 116023, Liaoning, Peoples R China 5.Dalian Glycobio Co Ltd, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Yunbin,Wang, Mengyu,Wang, Wenxia,et al. (1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,81:1069-1073. |
| APA | Fu, Yunbin.,Wang, Mengyu.,Wang, Wenxia.,Tuo, Yaqin.,Guo, Zhimou.,...&Yin, Heng.(2015).(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,81,1069-1073. |
| MLA | Fu, Yunbin,et al."(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 81(2015):1069-1073. |
入库方式： OAI收割
来源：大连化学物理研究所
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