Array-Based Two Dimensional Liquid Chromatography System for Proteomic Analysis of Human Plasma
文献类型：期刊论文
| 作者 | Huang Zhi1,2; Deng Nan3; Yan Guo-Quan1,2; Gao Ming-Xia1,2; Liang Zhen3; Zhang Li-Hua3; Zhang Xiang-Min1,2; Zhang Yu-Kui3 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 43期号:10页码:1472-1477 |
| 关键词 | Multidimensional Liquid Chromatography Array-based Column System High Abundance Protein Protein Equalizer Technology Human Plasma |
| ISSN号 | 0253-3820 |
| DOI | 10.1016/S1872-2040(15)60865-9 |
| 文献子类 | Article |
| 英文摘要 | The human plasma proteome has the characteristics of complexity in component and large dynamic range of protein concentrations. Herein, an array-based online two dimensional liquid chromatography system combined with protein equalizer technology was developed for the large-scale depletion of high abundance proteins and enrichment of low abundance proteins in human plasma. The array-based online two dimensional liquid chromatography system could be used to separate the plasma at the intact protein level with good reproducibility and high throughput. The total separation time was only 4 h and the fast location of high abundance proteins was also achieved. After the high abundance protein fractions was treated by ampholine@PM polymer microsphere, the number of identified low abundance proteins increased ten-fold, which significantly decreased the loss of low abundance proteins in high abundance protein fractions. The techniques combined were then applied to perform the proteomic analysis of human plasma sample. The total number of identified proteins was 1474 and the dynamic range of protein concentration was 7. In this work, 252 proteins were identified in high abundance protein fractions, among which 61 proteins belonged to high abundance proteins. These results demonstrated that an array-based online two dimensional liquid chromatography system combined with protein equalizer technology could efficiently achieve the large-scale depletion of high abundance proteins and the enrichment of low abundance proteins in human plasma, with a remarkable improvement in protein identification and a great prospect in the proteomic research of other complex samples. |
| WOS关键词 | HIGH-ABUNDANCE PROTEINS ; DYNAMIC-RANGE ; DEPLETION ; REMOVAL ; COLUMN |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000365738500003 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171588]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang Li-Hua |
| 作者单位 | 1.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 2.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang Zhi,Deng Nan,Yan Guo-Quan,et al. Array-Based Two Dimensional Liquid Chromatography System for Proteomic Analysis of Human Plasma[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(10):1472-1477. |
| APA | Huang Zhi.,Deng Nan.,Yan Guo-Quan.,Gao Ming-Xia.,Liang Zhen.,...&Zhang Yu-Kui.(2015).Array-Based Two Dimensional Liquid Chromatography System for Proteomic Analysis of Human Plasma.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(10),1472-1477. |
| MLA | Huang Zhi,et al."Array-Based Two Dimensional Liquid Chromatography System for Proteomic Analysis of Human Plasma".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.10(2015):1472-1477. |
入库方式： OAI收割
来源：大连化学物理研究所
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