Click Synthesis of Hydrophilic Maltose-Functionalized Iron Oxide Magnetic Nanoparticles Based on Dopamine Anchors for Highly Selective Enrichment of Glycopeptides
文献类型：期刊论文
| 作者 | Bi, Changfen2,5; Zhao, Yingran2,5; Shen, Lijin1,3; Zhang, Kai1,3; He, Xiwen2,5; Chen, Langxing2,5; Zhang, Yukui2,4,5 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2015-11-11 |
| 卷号 | 7期号:44页码:24670-24678 |
| 关键词 | Fe3o4 Nanoparticles Dopamine Copper(i)-catalyzed Azide-alkyne Cycloaddition (Cuaac) Maltose Glycopeptides Enrichment |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.5b06991 |
| 文献子类 | Article |
| 英文摘要 | The development of methods to isolate and enrich low-abundance glycopeptides from biological samples is crucial to glycoproteomics. Herein, we present an easy and one-step surface modification strategy to prepare hydrophilic maltose functionalized Fe3O4 nanoparticles (NPs). First, based on the chelation of the catechol ligand with iron atoms, azido-terminated dopamine (DA) derivative was assembled on the surface of magnetic Fe3O4 nanoparticles by sonication. Second, the hydrophilic maltose-functionalized Fe3O4 (Fe3O4-DA-Maltose) NPs were obtained via copper(I)-catalyzed azide-alkyne cycloaddition (click chemistry). The morphology, structure, and composition of Fe3O4-DA-Maltose NPs were investigated by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), X-ray photoelectron spectrometer (XPS), and vibrating sample magnetometer (VSM). Meanwhile, hydrophilicity of the obtained NPs was evaluated by water contact angle measurement. The hydrophilic Fe3O4-DA-Maltose NPs were applied in isolation and enrichment of glycopeptides from horseradish peroxidase (HRP), immunoglobulin (IgG) digests. The MALDI-TOF mass spectrometric analysis indicated that the novel NPs exhibited high detection sensitivity in enrichment from HRP digests at concentration as low as 0.05 ng mu L-1, a large binding capacity up to 43 mg g(-1), and good recovery for glycopeptides enrichment (85-110%). Moreover, the Fe3O4-DA-Maltose NPs were applied to enrich glycopeptides from human renal mesangial cells (HRMC) for identification of N-glycosylation sites. Finally, we identified 115 different N-linked glycopeptides, representing 93 gene products and 124 glycosylation sites in HRMC. |
| WOS关键词 | N-LINKED GLYCOPROTEINS ; DISTILLATION-PRECIPITATION POLYMERIZATION ; SOLID-PHASE EXTRACTION ; INTERACTION CHROMATOGRAPHY ; MASS-SPECTROMETRY ; GLYCOSYLATION HETEROGENEITY ; LIQUID-CHROMATOGRAPHY ; CHEMISTRY ; COMPOSITES ; DESIGN |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000364726400034 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171618]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Chen, Langxing |
| 作者单位 | 1.Tianjin Med Univ, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin 300070, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 5.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, Changfen,Zhao, Yingran,Shen, Lijin,et al. Click Synthesis of Hydrophilic Maltose-Functionalized Iron Oxide Magnetic Nanoparticles Based on Dopamine Anchors for Highly Selective Enrichment of Glycopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(44):24670-24678. |
| APA | Bi, Changfen.,Zhao, Yingran.,Shen, Lijin.,Zhang, Kai.,He, Xiwen.,...&Zhang, Yukui.(2015).Click Synthesis of Hydrophilic Maltose-Functionalized Iron Oxide Magnetic Nanoparticles Based on Dopamine Anchors for Highly Selective Enrichment of Glycopeptides.ACS APPLIED MATERIALS & INTERFACES,7(44),24670-24678. |
| MLA | Bi, Changfen,et al."Click Synthesis of Hydrophilic Maltose-Functionalized Iron Oxide Magnetic Nanoparticles Based on Dopamine Anchors for Highly Selective Enrichment of Glycopeptides".ACS APPLIED MATERIALS & INTERFACES 7.44(2015):24670-24678. |
入库方式： OAI收割
来源：大连化学物理研究所
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