A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy
文献类型：期刊论文
| 作者 | Qi, Qingkai1; Chi, Weijie2; Li, Yuanyuan3; Qiao, Qinglong1; Chen, Jie1; Miao, Lu1; Zhang, Yi1; Li, Jin1; Ji, Wei3; Xu, Tao3 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2019-05-14 |
| 卷号 | 10期号:18页码:4914-4922 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c9sc01284b |
| 通讯作者 | Xu, Tao(xutao@ibp.ac.cn) ; Liu, Xiaogang(xiaogang_liu@sutd.edu.sg) ; Yoon, Juyoung(jyoon@ewha.ac.kr) ; Xu, Zhaochao(zcxu@dicp.ac.cn) |
| 英文摘要 | Rhodamine spirolactam based photoswitches have been extensively applied in super-resolution single-molecule localization microscopy (SMLM). However, the ring-opening reactions of spirolactams are cross-sensitive to acid, limiting their photoswitch use to neutral pH conditions. In addition, the ring-closing reactions of spirolactams are environment-sensitive and slow (up to hours), virtually making rhodamine spirolactams caged fluorescent dyes instead of reversible photoswitches in SMLM. Herein, by introducing hydrogen bonds to stabilize spirolactams, we report a series of acid-resistant rhodamine spirolactams with accelerated ring-closing reactions from fluorescent xanthyliums to non-fluorescent spirolactams, endowing them with good photoswitchable properties even in acidic environments. By further substitution of 6-phenylethynyl naphthalimide on the spirolactam, we shifted the photoactivation wavelength into the visible region (>400 nm). Subsequently, we have successfully applied these dyes in labeling and imaging the cell surface of Bacillus subtilis at pH 4.5 using SMLM. |
| WOS关键词 | FLUORESCENCE NANOSCOPY ; DOMAINS ; APOPTOSIS ; CELLS ; DIARYLETHENES ; VISUALIZATION ; PHOTOCHROMISM |
| 资助项目 | National Natural Science Foundation of China[21708039] ; National Natural Science Foundation of China[21502189] ; National Natural Science Foundation of China[21878286] ; CPSF[2017M611278] ; CPSF[2018T110234] ; DICP[DMTO201603] ; DICP[TMSR201601] ; DICP[2015YB07] ; SUTD[T1SRCI17126] ; SUTD[IDD21700101] ; SUTD[IDG31800104] ; National Research Foundation of Korea (NRF)[2012R1A3A2048814] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000468818700020 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; CPSF ; CPSF ; DICP ; DICP ; SUTD ; SUTD ; National Research Foundation of Korea (NRF) ; National Research Foundation of Korea (NRF) |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171927]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xu, Tao; Liu, Xiaogang; Yoon, Juyoung; Xu, Zhaochao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Singapore Univ Technol & Design, 8 Somapah Rd, Singapore 487372, Singapore 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 4.Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea |
| 推荐引用方式 GB/T 7714 | Qi, Qingkai,Chi, Weijie,Li, Yuanyuan,et al. A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy[J]. CHEMICAL SCIENCE,2019,10(18):4914-4922. |
| APA | Qi, Qingkai.,Chi, Weijie.,Li, Yuanyuan.,Qiao, Qinglong.,Chen, Jie.,...&Xu, Zhaochao.(2019).A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy.CHEMICAL SCIENCE,10(18),4914-4922. |
| MLA | Qi, Qingkai,et al."A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy".CHEMICAL SCIENCE 10.18(2019):4914-4922. |
入库方式： OAI收割
来源：大连化学物理研究所
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