Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA silver staining in a tube
文献类型：期刊论文
| 作者 | Chen, Ziwei1; Duan, Xuying1; Wei, Hua1; Tang, Suming1; Xu, Chidong2 ; Li, Yanlei2; Guan, Yifu1; Zhao, Guojie1
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| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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| 出版日期 | 2018-05-01 |
| 卷号 | 50期号:5页码:507-515 |
| ISSN号 | 1672-9145 |
| 关键词 | galactose assay rolling circle amplification repressor silver staining |
| DOI | 10.1093/abbs/gmy034 |
| 英文摘要 | Oligonucleotides were screened for strongly silver-stained repetitive sequences. An 'AG'-clustered purine sequence showed strong staining, and the staining density can be compromised by disrupting the continuity of the 'AG'-clustered sequence. The staining-favored sequence was then employed in rolling circle amplification (RCA) for its product detection. A tube-staining method was developed for convenient and visual RCA assay. Moreover, by introducing GalR into RCA, D-galactose was detected by RCA tube-staining with naked eyes without any equipment. About 10 mM D-galactose can be easily identified, and the detection of D-galactose was specific in comparison with that of several other monosaccharides. |
| WOS关键词 | ROLLING CIRCLE AMPLIFICATION ; NUCLEIC-ACIDS ; POLYACRYLAMIDE-GELS ; GOLD NANOPARTICLES ; DNA ; PROTEINS ; STRATEGY ; BIOSENSOR |
| 资助项目 | National Natural Science Foundation of China[81772286] ; National Natural Science Foundation of China[81301517] ; National Natural Science Foundation of China[81371896] ; Anhui Province Key Laboratory of Medical Physics and Technology[LMPT201708] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000432042700010 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/35253]  |
| 专题 | 医学物理与技术中心 |
| 通讯作者 | Zhao, Guojie |
| 作者单位 | 1.China Med Univ, Dept Biochem & Mol Biol, Shenyang 110122, Liaoning, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ziwei,Duan, Xuying,Wei, Hua,et al. Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA silver staining in a tube[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2018,50(5):507-515. |
| APA | Chen, Ziwei.,Duan, Xuying.,Wei, Hua.,Tang, Suming.,Xu, Chidong.,...&Zhao, Guojie.(2018).Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA silver staining in a tube.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,50(5),507-515. |
| MLA | Chen, Ziwei,et al."Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA silver staining in a tube".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 50.5(2018):507-515. |
入库方式： OAI收割
来源：合肥物质科学研究院
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