Hyperspectral imaging (HSI) acquires a three-dimensional dataset called hypercube, with two spatial dimensions and one spectral dimension. HSI is an emerging imaging modality for various medical applications, especially in disease diagnosis of early and biomedical research based on their unique spectral signatures. A visible-near-infrared HSI for microspectroscopy is designed, the measured spectrum is from 450 nm to 900 nm, which is sampled by 256 spectral channels. The spatial resolution is 1.25μm.The modulation transfer function (MTF) value of full spectrum and full field of view is close to the diffraction limit. At last, this microspecroscopy have been fabricated and preliminary tests have been implemented. The results indicated this visible-near-infrared hyperspectral microscopy optical systems have excellent optical performances. This hyperspectral microscopy will be well developed and used in the life sciences fields. © 2019 SPIE.