Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State
文献类型：期刊论文
| 作者 | Liu DF7,8,10; Li X8,10; Jing JZ6,10; Du XM3,6,8,10; hongkui_deng@pku.edu.cn; zhenchai@pku.edu.cn; Deng HK*7,8,9; Ma YT5,8,10; Xu J8; Chai Z*6 |
| 刊名 | Cell Stem Cell
 |
| 出版日期 | 2017 |
| 卷号 | 21期号:X页码:1-10 |
| 英文摘要 | Direct lineage reprogramming, including with small molecules, has emerged as a promising approach for generating desired cell types. We recently found that during chemical induction of induced pluripotent stem cells (iPSCs) from mouse fibroblasts, cells pass through an extra-embryonic endoderm (XEN)-like state. Here, we show that these chemically induced XEN-like cells can also be induced to directly reprogram into functional neurons, bypassing the pluripotent state. The induced neurons possess neuron-specific expression profiles, form functional synapses in culture, and further mature after transplantation into the adult mouse brain. Using similar principles, we were also able to induce hepatocyte-like cells from the XEN-like cells. Cells in the induced XEN-like state were readily expandable over at least 20 passages and retained genome stability and lineage specification potential. Our study therefore establishes a multifunctional route for chemical lineage reprogramming and may provide a platform for generating a diverse range of cell types via application of this expandable XEN-like state. |
| 语种 | 英语 |
| 资助机构 | This work was supported by the National Basic Research Program of China (2015CB964501and2015CB964703),theNationalKeyResearchandDevelop- ment Program ofChina(2016YFA0100103),theNational Natural ScienceFoun- dation of China (31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Plan- ningProjectofGuangdongProvince,China(2014B020226001),andtheScience and Technology Program of Guangzhou, China (2016B030232001). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute. ; This work was supported by the National Basic Research Program of China (2015CB964501and2015CB964703),theNationalKeyResearchandDevelop- ment Program ofChina(2016YFA0100103),theNational Natural ScienceFoun- dation of China (31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Plan- ningProjectofGuangdongProvince,China(2014B020226001),andtheScience and Technology Program of Guangzhou, China (2016B030232001). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute. ; This work was supported by the National Basic Research Program of China (2015CB964501and2015CB964703),theNationalKeyResearchandDevelop- ment Program ofChina(2016YFA0100103),theNational Natural ScienceFoun- dation of China (31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Plan- ningProjectofGuangdongProvince,China(2014B020226001),andtheScience and Technology Program of Guangzhou, China (2016B030232001). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute. ; This work was supported by the National Basic Research Program of China (2015CB964501and2015CB964703),theNationalKeyResearchandDevelop- ment Program ofChina(2016YFA0100103),theNational Natural ScienceFoun- dation of China (31521004), the Guangdong Innovative and Entrepreneurial Research Team Program (2014ZT05S216), the Science and Technology Plan- ningProjectofGuangdongProvince,China(2014B020226001),andtheScience and Technology Program of Guangzhou, China (2016B030232001). This work was supported in part by a grant from the BeiHao Stem Cell and Regenerative Medicine Translational Research Institute. |
| 源URL | [http://159.226.149.26:8080/handle/152453/11769]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_哺乳动物胚胎发育 |
| 通讯作者 | hongkui_deng@pku.edu.cn; zhenchai@pku.edu.cn |
| 作者单位 | 1.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Health Science Center of Peking University, Beijing 100191, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Peking University-Tsinghua University-National Institute of Biological Science Joint Graduate Program, College of Life Science, Peking University, Beijing 100871, China 4.Laboratory of Molecular Iron Metabolism, College of Life Science, Hebei Normal University, Shijiazhuang 050024, China 5.Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China 6.State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China 7.Shenzhen Stem Cell Engineering Laboratory, Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen, Guangdong 518055, China 8.Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China 9.Lead Contact 10.These authors contributed equally |
| 推荐引用方式 GB/T 7714 | Liu DF,Li X,Jing JZ,et al. Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State[J]. Cell Stem Cell,2017,21(X):1-10. |
| APA | Liu DF.,Li X.,Jing JZ.,Du XM.,hongkui_deng@pku.edu.cn.,...&Wang LP.(2017).Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State.Cell Stem Cell,21(X),1-10. |
| MLA | Liu DF,et al."Direct Reprogramming of Fibroblasts via a Chemically Induced XEN-like State".Cell Stem Cell 21.X(2017):1-10. |
入库方式： OAI收割
来源：昆明动物研究所
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