Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
文献类型：期刊论文
| 作者 | Ian.Brennan@anu.edu.au; Brennan IG*6; Bauer AN6; Zhang YP1,3; Murphy RW1,2; Ngo VT5; Wang YY1; Wang WZ1,4 |
| 刊名 | Scientific Reports
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:X页码:e5592 |
| 英文摘要 | Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the utility of barcoding data for the taxonomic group of interest. Here, our primary aim is to determine the phylogenetic value of DNA barcoding (mitochondrial locus COI) within the gecko genus Cyrtodactylus. With >40 new species described since last systematic investigation, Cyrtodactylus represents one of the most diverse extant squamate genera, and their contemporary distribution spans the Indian subcontinent, eastward through Indochina, and into AustraloPapua. The complex biogeographic history of this group, and morphology-only designation of many species have complicated our phylogenetic understanding of Cyrtodactylus. To highlight the need for continued inclusive molecular assessment, we use Vietnamese Cyrtodactylus as a case study showing the geopolitically paraphyletic nature of their history. We compare COI to the legacy marker ND2, and discuss the value of COI as an interspecific marker, as well as its shortcomings at deeper evolutionary scales. We draw attention back to the Cold Code as a subsidized method for incorporating molecular methods into species descriptions in the effort to maintain accurate phylogenies. |
| 语种 | 英语 |
| 资助机构 | This work was supported by the National Natural Science Foundation of China (NSFC grant no. 31090251 to Y.Z.), the Ministry of Science and Technology of China (MOST no. 2012FY110800 to W.W.), and the Joint Grant of CAS Kunming Branch and Guizhou Academy of Sciences (CASKMB no. 2014-001 to W.W.). This work was also supported by the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences (the Large Research Infrastructure Funding, Funding code: GBOWS to W.W. and Y.Z.), an Australian American Association Fellowship to I.G.B, and National Science Foundation (USA) grants DEB 0844523 and EF 1241885 (sub-award 13-0632) to A.M.B. ; This work was supported by the National Natural Science Foundation of China (NSFC grant no. 31090251 to Y.Z.), the Ministry of Science and Technology of China (MOST no. 2012FY110800 to W.W.), and the Joint Grant of CAS Kunming Branch and Guizhou Academy of Sciences (CASKMB no. 2014-001 to W.W.). This work was also supported by the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences (the Large Research Infrastructure Funding, Funding code: GBOWS to W.W. and Y.Z.), an Australian American Association Fellowship to I.G.B, and National Science Foundation (USA) grants DEB 0844523 and EF 1241885 (sub-award 13-0632) to A.M.B. ; This work was supported by the National Natural Science Foundation of China (NSFC grant no. 31090251 to Y.Z.), the Ministry of Science and Technology of China (MOST no. 2012FY110800 to W.W.), and the Joint Grant of CAS Kunming Branch and Guizhou Academy of Sciences (CASKMB no. 2014-001 to W.W.). This work was also supported by the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences (the Large Research Infrastructure Funding, Funding code: GBOWS to W.W. and Y.Z.), an Australian American Association Fellowship to I.G.B, and National Science Foundation (USA) grants DEB 0844523 and EF 1241885 (sub-award 13-0632) to A.M.B. ; This work was supported by the National Natural Science Foundation of China (NSFC grant no. 31090251 to Y.Z.), the Ministry of Science and Technology of China (MOST no. 2012FY110800 to W.W.), and the Joint Grant of CAS Kunming Branch and Guizhou Academy of Sciences (CASKMB no. 2014-001 to W.W.). This work was also supported by the Animal Branch of the Germplasm Bank of Wild Species, Chinese Academy of Sciences (the Large Research Infrastructure Funding, Funding code: GBOWS to W.W. and Y.Z.), an Australian American Association Fellowship to I.G.B, and National Science Foundation (USA) grants DEB 0844523 and EF 1241885 (sub-award 13-0632) to A.M.B. |
| 源URL | [http://159.226.149.26:8080/handle/152453/11939]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_分子进化基因组学 昆明动物研究所_生命条形码中心 |
| 通讯作者 | Ian.Brennan@anu.edu.au |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 2.Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, M5S 2C6, Canada 3.Laboratory for Conservation and Utilization of Bio-resource and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, 2 Cuihu N. Rd., Kunming, 650091, China 4.South China DNA Barcoding Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China 5.National Key Laboratory, Institute of Tropical Biology, Vietnamese Academy of Sciences and Technology, 9/621 Hanoi Highway, Linh Trung District, Ho Chi Minh City, Vietnam 6.Division of Ecology & Evolution, Research School of Biology, The Australian National University, Canberra, ACT 2602, Australia |
| 推荐引用方式 GB/T 7714 | Ian.Brennan@anu.edu.au,Brennan IG*,Bauer AN,et al. Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos[J]. Scientific Reports,2017,7(X):e5592. |
| APA | Ian.Brennan@anu.edu.au.,Brennan IG*.,Bauer AN.,Zhang YP.,Murphy RW.,...&Wang WZ.(2017).Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos.Scientific Reports,7(X),e5592. |
| MLA | Ian.Brennan@anu.edu.au,et al."Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos".Scientific Reports 7.X(2017):e5592. |
入库方式： OAI收割
来源：昆明动物研究所
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