A high concentration of DMSO activates caspase-1 by increasing the cell membrane permeability of potassium
文献类型：期刊论文
| 作者 | Sun SJ1,4; Zhang Y*4; Guo XL4; Zhao MM3,4; xiangyang@ncu.edu.cn; zhangy@mail.kiz.ac.cn; Lee WH4; Li SA4; Wang QQ3,4; Xiang Y*2,4 |
| 刊名 | Cytotechnology
 |
| 出版日期 | 2017 |
| 卷号 | **期号:**页码:published online |
| 关键词 | Dmso Nlrp3 Inflammasome Potassium Efflux Interleukin-1b |
| 英文摘要 | Dimethyl sulfoxide (DMSO) is widely used in the laboratory and in clinical situations because it is soluble in both aqueous and organic media and can be used to treat many types of diseases. Thus, it is meaningful to assess the comprehensive and in-depth biological activities of DMSO. Here, we showed that a high concentration of DMSO induced pro-inflammatory cytokine interleukin-1β (IL-1β) secretion from the monocytic cell line THP-1. DMSO-induced IL-1β secretion was dependent on intracellular caspase-1 activation. Further study revealed that the activation of caspase-1 by DMSO relied on NLRP3 inflammasome formation. It is generally accepted that the NLRP3 inflammasome is activated by reactive oxygen species generation or potassium efflux; however, the common NLRP3 inflammasome trigger remains controversial. Here, we showed that although DMSO is a ROS scavenger, this chemical increases membrane permeability and potassium efflux, and the formation of the NLRP3 inflammasome reflects the increased membrane permeability and potassium efflux induced by DMSO. The present study reveals a new characteristic of DMSO, which should be considered when using this chemical in either the laboratory or the clinic. |
| 语种 | 英语 |
| 资助机构 | This work was financially supported by the National Natural Science Foundation of China to Yang Xiang (91649120) and Sujiao Sun (81560697), Applied and basic research project of Yunnan Province (2014FB175) to Yang Xiang, and ‘‘Yunnan scholar’’ Program to Yun Zhang. ; This work was financially supported by the National Natural Science Foundation of China to Yang Xiang (91649120) and Sujiao Sun (81560697), Applied and basic research project of Yunnan Province (2014FB175) to Yang Xiang, and ‘‘Yunnan scholar’’ Program to Yun Zhang. ; This work was financially supported by the National Natural Science Foundation of China to Yang Xiang (91649120) and Sujiao Sun (81560697), Applied and basic research project of Yunnan Province (2014FB175) to Yang Xiang, and ‘‘Yunnan scholar’’ Program to Yun Zhang. ; This work was financially supported by the National Natural Science Foundation of China to Yang Xiang (91649120) and Sujiao Sun (81560697), Applied and basic research project of Yunnan Province (2014FB175) to Yang Xiang, and ‘‘Yunnan scholar’’ Program to Yun Zhang. |
| 源URL | [http://159.226.149.26:8080/handle/152453/12054]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物活性蛋白多肽组学 |
| 通讯作者 | xiangyang@ncu.edu.cn; zhangy@mail.kiz.ac.cn |
| 作者单位 | 1.Medical Cosmetology Teaching and Research Section, Dali University School of Clinical Medicine, Jiashibo Road 32, Dali, Yunnan Province 671000, China 2.Human Aging Research Institute and School of Life Sciences, Nanchang University, Nanchang, China 3.Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China 4.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China |
| 推荐引用方式 GB/T 7714 | Sun SJ,Zhang Y*,Guo XL,et al. A high concentration of DMSO activates caspase-1 by increasing the cell membrane permeability of potassium[J]. Cytotechnology,2017,**(**):published online. |
| APA | Sun SJ.,Zhang Y*.,Guo XL.,Zhao MM.,xiangyang@ncu.edu.cn.,...&Xiang Y*.(2017).A high concentration of DMSO activates caspase-1 by increasing the cell membrane permeability of potassium.Cytotechnology,**(**),published online. |
| MLA | Sun SJ,et al."A high concentration of DMSO activates caspase-1 by increasing the cell membrane permeability of potassium".Cytotechnology **.**(2017):published online. |
入库方式： OAI收割
来源：昆明动物研究所
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