Proteotranscriptomic Analysis and Discovery of the Profile and Diversity of Toxin-like Proteins in Centipede
文献类型:期刊论文
作者 | Feng Zhao1,2,3; Yun Zhang3; Tao Li1,2; Wen- Hui Lee3; Yang Xiang3; Fang Zhao1,2 |
刊名 | Molecular & Cellular Proteomics |
出版日期 | 2018 |
卷号 | 17期号:4页码:709-720 |
关键词 | Centipede Toxins Peptidomics Gene Recruitment |
DOI | 10.1074/mcp.RA117.000431 |
英文摘要 | Centipedes are one of the oldest venomous animals and use their venoms as weapons to attack prey orprotect themselves. Their venoms contain various components with different biomedical andpharmacological properties. However, little attention has been paid to the profiles and diversity of theirtoxin-like proteins/peptides. In this study, we used a proteotranscriptomic approach to uncover thediversity of centipede toxin-like proteins in Scolopendra subspinipes mutilans. Nine hundred twenty-three and 6,736 peptides, which were separately isolated from venom and torso tissues, respectively, wereidentified by ESI-MS/MS and deduced from their transcriptomes. Finally, 1,369 unique proteins wereidentified in the proteome, including 100 proteins that exhibited overlapping expression in venom andtorso tissues. Of these proteins, at least 40 proteins were identified as venom toxin-like proteins.Meanwhile, transcriptome mining identified approximately 10-fold more toxin-like proteins and enabledthe characterization of the precursor architecture of mature toxin-like peptides. Importantly, combinedwith proteomic and transcriptomic analyses, 25 toxin-like proteins/peptides (neurotoxins accounted for50%) were expressed outside the venom gland and involved in gene recruitment processes. Thesefindings highlight the extensive diversity of centipede toxin-like proteins and provide a new foundationfor the medical-pharmaceutical use of centipede toxin-like proteins. Moreover, we are the first group toreport the gene recruitment activity of venom toxin-like proteins in centipede, similar to snakes. |
语种 | 英语 |
源URL | [http://159.226.149.26:8080/handle/152453/12395] |
专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
通讯作者 | Yang Xiang; Fang Zhao |
作者单位 | 1.Institute of Comparative Study of Traditional Materia Medica, Institute of Integrative Medicine of Fudan University, Shanghai China 2.Key Laboratory of Subtropical Medicinal Edible Resources Development and Utilization in Yunnan Province, Department of Biology and Chemistry, Puer University, 6 Xueyuan Road, Puer, Yunnan 665000, China 3.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao-Chang Road, Kunming, Yunnan 650223, China 4.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China |
推荐引用方式 GB/T 7714 | Feng Zhao,Yun Zhang,Tao Li,et al. Proteotranscriptomic Analysis and Discovery of the Profile and Diversity of Toxin-like Proteins in Centipede[J]. Molecular & Cellular Proteomics,2018,17(4):709-720. |
APA | Feng Zhao,Yun Zhang,Tao Li,Wen- Hui Lee,Yang Xiang,&Fang Zhao.(2018).Proteotranscriptomic Analysis and Discovery of the Profile and Diversity of Toxin-like Proteins in Centipede.Molecular & Cellular Proteomics,17(4),709-720. |
MLA | Feng Zhao,et al."Proteotranscriptomic Analysis and Discovery of the Profile and Diversity of Toxin-like Proteins in Centipede".Molecular & Cellular Proteomics 17.4(2018):709-720. |
入库方式: OAI收割
来源:昆明动物研究所
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