Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea
文献类型：期刊论文
| 作者 | Wang, QL (Wang, Qiuli)1,2,3,4; Pu, YA (Pu, Yanan)1,2,3,5; Yang, DN (Yang, Danni)1,2,3,5; Yin, X (Yin, Xin)1,2,3,5; He, ZR (He, Zhaorong)4; Yang, YQ (Yang, Yunqiang)1,2,3; Yang, YP (Yang, Yongping)1,2,3 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2018-01-08 |
| 卷号 | 495期号:2页码:1851-1857 |
| 关键词 | EXPRESSION ANALYSIS ANTIOXIDANT SYSTEMS HYDROGEN-PEROXIDE OXIDATIVE STRESS SALT STRESS OXYGEN TOLERANCE ASCORBATE RESPONSES ENZYMES |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2017.12.054 |
| 英文摘要 | Reactive oxygen species (ROS) are a key factor in abiotic stresses; excess ROS is harmful to plants. Glutathione reductase (GR) plays an important role in scavenging ROS in plants. Here, a GR gene, named SpGR, was cloned from Stipa purpurea and characterized. The full-length open reading frame was 1497 bp, encoding 498 amino acids. Subcellular localization analysis indicated that SpGR was localized to both the plasma membrane and nucleus. The expression of SpGR was induced by cold, salt, and drought stresses. Functional analysis indicated that ectopic expression of SpGR in Arabidopsis thaliana resulted in greater tolerance to salt stress than that of wild-type plants, but no difference under cold or drought treatments. The results of GR activity and GSSG and GSH content analyses suggested that, under salt stress, transgenic plants produced more GR to reduce GSSG to GSH for scavenging ROS than wild-type plants. Therefore, SpGR may be a candidate gene for plants to resist abiotic stress. (C) 2017 Elsevier Inc. All rights reserved. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000424732700042 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.itpcas.ac.cn/handle/131C11/8763]  |
| 专题 | 青藏高原研究所_图书馆 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China; 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China; 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China; 4.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Wang, QL ,Pu, YA ,Yang, DN ,et al. Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(2):1851-1857. |
| APA | Wang, QL .,Pu, YA .,Yang, DN .,Yin, X .,He, ZR .,...&Yang, YP .(2018).Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(2),1851-1857. |
| MLA | Wang, QL ,et al."Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.2(2018):1851-1857. |
入库方式： OAI收割
来源：青藏高原研究所
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