MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN
文献类型：期刊论文
| 作者 | Fayyaz, Amna1; Tufail, Muhammad Rizwan1; Sahi, Shahbaz Talib1; Masood, Atifa2; Tazeen, Syeda Khola2; Naseer, Ignaa3; Farooq, Taimoor Hassan4; Amrao, Luqman1 |
| 刊名 | FRESENIUS ENVIRONMENTAL BULLETIN
 |
| 出版日期 | 2019 |
| 卷号 | 28期号:3页码:1770-1774 |
| 关键词 | Chili Leaf Curl Disease Begomoviruses Chili Leaf Curl Virus Betasatellite RFLP |
| ISSN号 | 1018-4619 |
| 通讯作者 | Masood, Atifa(dratifabot@yahoo.com) |
| 英文摘要 | Among many other plant diseases Chili Leaf Curl Disease (ChLCD) caused by Begomovirus complex is a crucial threat for chili worldwide. Chili Leaf Curl Virus (ChLCV) is monopartites DNA Begomovirus having DNA-A which comprises of 2754 nucleotides having six ORFs and betasetallite of size 1.3kb. Genomic DNA of infected chili leaf samples was extracted by CTAB method, collected from different field areas of Faisalabad. Circular molecules from genomic DNA were amplified by using Rolling Circle Amplification (RCA). Three samples showed successful RCA which further used in PCR amplification. For amplification of full length Begomovirus from RCA product universal primers were used. Betasatellite component associated with ChLCD was amplified with the help of PCR by using full-length universal primers 0301 and 002). RFLP analysis showed that single type of Begomovirus and diverse type of betasatellite molecules observed in infected chili samples. |
| WOS关键词 | 1ST REPORT ; GENETIC DIVERSITY ; DNA-BETA ; BEGOMOVIRUS ; DISEASE ; TOMATO ; PEPPER ; VECTOR |
| 资助项目 | Higher Education Commission, Pakistan |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000463297500015 |
| 出版者 | PARLAR SCIENTIFIC PUBLICATIONS (P S P) |
| 资助机构 | Higher Education Commission, Pakistan |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/48201]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Masood, Atifa |
| 作者单位 | 1.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan 2.Univ Lahore, Dept Bot, Sargodha, Punjab, Pakistan 3.Univ Chinese Acad Sci, Inst Geog Sci & Nat Resources, Key Lab Ecosyst Res Network Observat & Modeling, Beijing, Peoples R China 4.Fujian Agr & Forestry Univ, Forestry & Environm Res Inst, Fuzhou, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fayyaz, Amna,Tufail, Muhammad Rizwan,Sahi, Shahbaz Talib,et al. MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2019,28(3):1770-1774. |
| APA | Fayyaz, Amna.,Tufail, Muhammad Rizwan.,Sahi, Shahbaz Talib.,Masood, Atifa.,Tazeen, Syeda Khola.,...&Amrao, Luqman.(2019).MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN.FRESENIUS ENVIRONMENTAL BULLETIN,28(3),1770-1774. |
| MLA | Fayyaz, Amna,et al."MOLECULAR CHARACTERIZATION AND EMERGENCE OF CHILI LEAF CURL VIRUS (CHLCV) INFECTING CHILI CROPS IN PAKISTAN".FRESENIUS ENVIRONMENTAL BULLETIN 28.3(2019):1770-1774. |
入库方式： OAI收割
来源：地理科学与资源研究所
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