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High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

文献类型：期刊论文

作者Yin, Lina1,2,3; Mano, Jun'ichi4,5; Tanaka, Kiyoshi3; Wang, Shiwen1,2; Zhang, Meijuan1; Deng, Xiping1,2; Zhang, Suiqi1,2
刊名PHYSIOLOGIA PLANTARUM
出版日期2017-10-01
卷号161期号:2页码:211-223
ISSN号0031-9317
DOI10.1111/ppl.12583
通讯作者Yin, Lina(linayin@nwsuaf.edu.cn)
英文摘要Lipid peroxide-derived reactive carbonyl species (RCS), generated downstream of reactive oxygen species (ROS), are critical damage-inducing species in plant aluminum (Al) toxicity. In mammals, RCS are scavenged primarily by glutathione (reduced form of glutathione, GSH), but in plant Al stress, contribution of GSH to RCS detoxification has not been evaluated. In this study, Arabidopsis plants overexpressing the gene AtGR1 (accession code At3g24170), encoding glutathione reductase (GR), were generated, and their performance under Al stress was examined. These transgenic plants (GR-OE plants) showed higher GSH levels and GSH/GSSG (oxidized form of GSH) ratio, and an improved Al tolerance as they suffered less inhibition of root growth than wild-type under Al stress. Exogenous application of 4-hydroxy-2-nonenal, an RCS responsible for Al toxicity in roots, markedly inhibited root growth in wild-type plants. GR-OE plants suffered significantly smaller inhibition, indicating that the enhanced GSH level increased the capacity of RCS detoxification. The generation of H2O2 due to Al stress in GR-OE plants was lower by 26% than in wild-type. Levels of various RCS, such as malondialdehyde, butyraldehyde, phenylacetaldehyde, (E)-2-heptenal and n-octanal, were suppressed by more than 50%. These results indicate that high levels of GSH and GSH/GSSG ratio by GR overexpression contributed to the suppression of not only ROS, but also RCS. Thus, the maintenance of GSH level by overexpressing GR reinforces dual detoxification functions in plants and is an efficient approach to enhance Al tolerance.
WOS关键词WHEAT TRITICUM-AESTIVUM ; OXIDATIVE STRESS ; SUPEROXIDE-DISMUTASE ; PHOTOOXIDATIVE STRESS ; SENSITIVE METHOD ; TOLERANCE ; PLANTS ; RESISTANCE ; CHLOROPLASTS ; TOXICITY
资助项目National Natural Sciences Foundation of China[31200206] ; Northwest AF University ; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau[A314021403-C5] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2015389] ; West Light Foundation of the Chinese Academy of Sciences
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000417402000003
出版者WILEY
资助机构National Natural Sciences Foundation of China ; Northwest AF University ; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; West Light Foundation of the Chinese Academy of Sciences
源URL[http://ir.igsnrr.ac.cn/handle/311030/56794]  
专题中国科学院地理科学与资源研究所
通讯作者Yin, Lina
作者单位1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
3.Tottori Univ, Fac Agr, Tottori 6808533, Japan
4.Yamaguchi Univ, Sci Res Ctr, Yamaguchi 7538515, Japan
5.Yamaguchi Univ, Grad Sch Agr, Yamaguchi 7538515, Japan
推荐引用方式
GB/T 7714		 Yin, Lina,Mano, Jun'ichi,Tanaka, Kiyoshi,et al. High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress[J]. PHYSIOLOGIA PLANTARUM,2017,161(2):211-223.
APA Yin, Lina.,Mano, Jun'ichi.,Tanaka, Kiyoshi.,Wang, Shiwen.,Zhang, Meijuan.,...&Zhang, Suiqi.(2017).High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress.PHYSIOLOGIA PLANTARUM,161(2),211-223.
MLA Yin, Lina,et al."High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress".PHYSIOLOGIA PLANTARUM 161.2(2017):211-223.

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来源：地理科学与资源研究所

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