Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure
文献类型：期刊论文
| 作者 | Liu, Zhen-wen1; Gao, Yu-zhen2,3; Zhou, Jing2,3 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2019-04-09 |
| 卷号 | 10页码:8 |
| 关键词 | apiaceae DNA barcoding Ligustici Rhizoma et Radix ITS2 secondary structures |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2019.00429 |
| 通讯作者 | Zhou, Jing(zhoujing_apiaceae@163.com) |
| 英文摘要 | Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as "Gaoben," refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as "Gao-ben" in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The "Gao-ben" is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true "Gaoben" and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine. |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000463915400001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/66164]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 通讯作者 | Zhou, Jing |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China 2.Kunming Med Univ, Sch Pharmaceut Sci, Kunming, Yunnan, Peoples R China 3.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhen-wen,Gao, Yu-zhen,Zhou, Jing. Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure[J]. FRONTIERS IN PLANT SCIENCE,2019,10:8. |
| APA | Liu, Zhen-wen,Gao, Yu-zhen,&Zhou, Jing.(2019).Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure.FRONTIERS IN PLANT SCIENCE,10,8. |
| MLA | Liu, Zhen-wen,et al."Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure".FRONTIERS IN PLANT SCIENCE 10(2019):8. |
入库方式： OAI收割
来源：昆明植物研究所
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