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| 作者 | Fengmin Li; Fuyun Li; Xiaodong Hou; Xianxiang Luo; Haifeng Tu; Yadan Zou; Cuizhu Sun; Mei Shi; Hao Zheng
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| 刊名 | Marine Pollution Bulletin
 ; Marine Pollution Bulletin
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| 出版日期 | 2018
; 2018
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| 卷号 | 131页码:515–524 |
| 关键词 | Fluorescent Ps Beads
Fluorescent Ps Beads
Digestion
Fluorescence Intensity
Morphology
Extraction Efficiency
Digestion
Fluorescence Intensity
Morphology
Extraction Efficiency
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| 英文摘要 |
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Effect of digestion methods on fluorescence intensity of fluorescent polystyrene (PS) beads was poorly understood, which may affect the accuracy of toxicity test of the fluorescent PS beads exposed to marine organisms. Therefore, six digestion approaches were compared on fluorescence intensities and properties of three commercial fluorescent PS beads. Among all the protocols, the digestion using KOH (10% w/v, 60 degrees C) (KOH-digestion) had no effect on the fluorescence intensity, morphology and composition of the three fluorescent PS beads. Moreover, the extraction efficiency >= 95.3 0.2% of fluorescent PS beads in Daphnia magna and zebrafish, confirming its feasibility in fluorescent PS beads quantitative analysis. However, the fluorescence intensities of fluorescent PS beads digested by other five protocols were significantly decreased, as well as the change of morphology and composition on fluorescent PS beads. Overall, the KOH-digestion is an optimal protocol for extracting fluorescent PS beads in biological samples.
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Effect of digestion methods on fluorescence intensity of fluorescent polystyrene (PS) beads was poorly understood, which may affect the accuracy of toxicity test of the fluorescent PS beads exposed to marine organisms. Therefore, six digestion approaches were compared on fluorescence intensities and properties of three commercial fluorescent PS beads. Among all the protocols, the digestion using KOH (10% w/v, 60 degrees C) (KOH-digestion) had no effect on the fluorescence intensity, morphology and composition of the three fluorescent PS beads. Moreover, the extraction efficiency >= 95.3 0.2% of fluorescent PS beads in Daphnia magna and zebrafish, confirming its feasibility in fluorescent PS beads quantitative analysis. However, the fluorescence intensities of fluorescent PS beads digested by other five protocols were significantly decreased, as well as the change of morphology and composition on fluorescent PS beads. Overall, the KOH-digestion is an optimal protocol for extracting fluorescent PS beads in biological samples.
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| 语种 | 英语
; 英语
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| 源URL | [http://ir.gyig.ac.cn/handle/42920512-1/8818]  |
| 专题 | 地球化学研究所_环境地球化学国家重点实验室
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| 作者单位 | 1.Institute of Coastal Environmental Pollution Control, and Ministry of Education Key Laboratory of Marine Environment and Ecology, Ocean University of China, Qingdao 266100, China
2.College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
3.Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550000, China
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推荐引用方式
GB/T 7714 |
Fengmin Li,Fuyun Li,Xiaodong Hou,et al. Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads, Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads[J]. Marine Pollution Bulletin, Marine Pollution Bulletin,2018, 2018,131, 131:515–524, 515–524.
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| APA |
Fengmin Li.,Fuyun Li.,Xiaodong Hou.,Xianxiang Luo.,Haifeng Tu.,...&Hao Zheng.(2018).Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads.Marine Pollution Bulletin,131,515–524.
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| MLA |
Fengmin Li,et al."Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads".Marine Pollution Bulletin 131(2018):515–524.
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