The pollution of natural waters and sediments with metals derived from acid mine drainage (AMD) is a global environmental problem. However, the processes governing the transportation and transformation of AMD metals such as Cd in mountainous areas are poorly understood. In this study, the Cd isotopic composition and Cd concentration of river water and sediments (16 sampling sites) from an AMD-affected river in southern China were determined. Cd concentration in river water declined from its source at a tailings dam (304 mu g L-1) to a point 14 km downstream (0.32 mu g L-1). Sediment Cd concentration ranged from 0.18 to 39.9 mu g g(-1), suggesting that anthropogenic Cd is derived primarily from the tailing dam and easily enters the solid phase of the river. Isotopic data showed that the dissolved Cd in rivers was characterized by delta Cd-114/110 values ranging from 0.21% to 1.03%, with a mean of 0.48%. The greatest Cd isotope difference was observed between the water and sediments in the LW dam (Delta Cd-114/110(river-sediment) = 1.61 parts per thousand, site 1), likely due to a rapid weathering dissolution of the ore tailings. In the river's upper reach (sites 2-3), isotope difference between river and sediment (Delta Cd-114/110(river-sediment)) ranged from 1.0% to 0.91 parts per thousand. This suggests that a host of secondary processes might have impacted Cd isotope fractionation, including adsorption, ternary complexation and/or (co) precipitation of Cd on secondary oxides and hydroxides. In the middle and lower reaches, an abruptly elevated delta Cd-114/110 value near farmland (site 10) suggests the existence of a second Cd source. Based on the chemical properties of water samples we can attribute this heavy isotope signature to agricultural fertilizer and drainage from agricultural fields. Our results suggest that Cd isotope is a tracer for identifying and tracking Cd sources and attenuation mechanisms (adsorption/(co) precipitation) in a complex mountain watershed.