Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance
文献类型：期刊论文
| 作者 | Jia, Dongjie1; Fan, Lianmei1; Shen, Junling1; Qin, Song2; Li, Fuchao2; Yuan, Yongbing1 |
| 刊名 | SCIENTIA HORTICULTURAE
 |
| 出版日期 | 2019-01-03 |
| 卷号 | 243页码:428-433 |
| 关键词 | Malus domestica Borkh Astaxanthin beta-Carotene ketolase beta-Carotene hydroxylase Photoprotection |
| ISSN号 | 0304-4238 |
| DOI | 10.1016/j.scienta.2018.08.058 |
| 通讯作者 | Yuan, Yongbing(yyb@qau.edu.cn) |
| 英文摘要 | Apples may suffer sunburn during growing season because of photooxidation damage. Astaxanthin have a stronger antioxidant activity against photooxidation compared to its precursors zeaxanthin and beta-carotene. Here, we transferred beta-carotene ketolase (bkt) and beta-carotene hydroxylase (crtR-B), two genes encoding key enzymes for astaxanthin biosynthesis, into "Brookfield Gala" apple plants using pCAMBIA1302-bkt-crtR-B via transformation mediated by Agrobacterium tumefaciens EHA105. We aimed to confer a stronger antioxidant ability against sunburn to apple plants by regulating metabolic pathways of carotenoids. RT-PCR and qPCR analysis confirmed that bkt and crtR-B genes were expressed simultaneously in transgenic plants. Southern blotting analysis confirmed that the transgene was stably integrated and determined transgene copy number. Transgenic plants synthesized novel ketocarotenoids, resulting in an accumulation of astaxanthin up to 12.06 mu g/g and of canthaxanthin up to 6.38 mu g/g per leaf fresh weight. The light antioxidant ability of transgenic plantlets was stronger than that of non-transgenic ones in high light conditions. We speculate that the transgenic apples accumulating astaxanthin are resistant to photooxidation and can therefore prevent sunburn. |
| 资助项目 | National Key Research and Development Program of China[2016YFD0400100] ; project of key agricultural technique research of Shandong Province ; [NSFC30471200] |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000450381800053 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/156385]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Yuan, Yongbing |
| 作者单位 | 1.Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Dongjie,Fan, Lianmei,Shen, Junling,et al. Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance[J]. SCIENTIA HORTICULTURAE,2019,243:428-433. |
| APA | Jia, Dongjie,Fan, Lianmei,Shen, Junling,Qin, Song,Li, Fuchao,&Yuan, Yongbing.(2019).Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance.SCIENTIA HORTICULTURAE,243,428-433. |
| MLA | Jia, Dongjie,et al."Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance".SCIENTIA HORTICULTURAE 243(2019):428-433. |
入库方式： OAI收割
来源：海洋研究所
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