Development of microsatellite markers for the seagrass Zostera japonica using next-generation sequencing
文献类型：期刊论文
| 作者 | Zhang, Xiaomei1,2,3 ; Zhou, Yi1,2,3; Li, Yu-Long1,2,3; Liu, Jin-Xian1,2,3
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| 刊名 | MOLECULAR BIOLOGY REPORTS
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| 出版日期 | 2019-02-01 |
| 卷号 | 46期号:1页码:1335-1341 |
| 关键词 | Zostera japonica NGS Microsatellites Population genetics |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-018-4491-2 |
| 通讯作者 | Zhou, Yi(yizhou@qdio.ac.cn) ; Liu, Jin-Xian(jxliu@qdio.ac.cn) |
| 英文摘要 | The seagrass Zostera japonica Asch. & Graebn. is among the few seagrass species that thrive both in temperate and tropical intertidal zones. The degradation of Z. japonica in its native range and continued expansion in some localities have raised concerns with regard to its conservation and management. Population genetic studies of Z. japonica across its biogeographic range and within meadows are scarce. Previously developed microsatellites via traditional methods are not sufficient in quantity and perform inconsistently between the temperate and tropical populations. Thus, this study aimed to develop more reliable polymorphic markers for Z. japonica using next generation sequencing. In total, 6479 sequences containing loci were identified and 1619 pairs of primers were successfully designed. Of these, 63 loci were selected for primary validation in 16 individuals from four populations, with 48 (76.2%) polymorphic loci detected. Seventeen polymorphic loci were selected for further evaluation in 62 individuals from one temperate and one tropical population. The overall probability of identity (PID) for both populations was 2.375e(-22) (PIDsib=3.557e(-08)). The number of alleles, and expected and observed heterozygosity in the two populations were both relatively high and not significantly different from each other. The pairwise F-ST=0.232 (p<0.0001) and Principal Coordinates Analysis both suggested a large and significant genetic divergence between the two populations. This study makes abundant molecular markers available for genetic studies in Z. japonica, and facilitates its conservation and management strategies. |
| 资助项目 | National Science & Technology Basic Work Program[2015FY110600] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows[2016LH0032] ; China Postdoctoral Science Foundation[2016M600562] ; key Research and Development Project of Shandong Province[2017GHY15111] ; Key Research Project of Frontier Sciences of CAS[QYZDB-SSW-DQC041-1] ; Science and Technology Service Network Initiative of CAS[KFJ-STS-ZDTP-023] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science from the Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; Taishan Scholars Program (Distinguished Taishan Scholars) |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000462022300132 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/154929]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhou, Yi; Liu, Jin-Xian |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaomei,Zhou, Yi,Li, Yu-Long,et al. Development of microsatellite markers for the seagrass Zostera japonica using next-generation sequencing[J]. MOLECULAR BIOLOGY REPORTS,2019,46(1):1335-1341. |
| APA | Zhang, Xiaomei,Zhou, Yi,Li, Yu-Long,&Liu, Jin-Xian.(2019).Development of microsatellite markers for the seagrass Zostera japonica using next-generation sequencing.MOLECULAR BIOLOGY REPORTS,46(1),1335-1341. |
| MLA | Zhang, Xiaomei,et al."Development of microsatellite markers for the seagrass Zostera japonica using next-generation sequencing".MOLECULAR BIOLOGY REPORTS 46.1(2019):1335-1341. |
入库方式： OAI收割
来源：海洋研究所
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