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The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses

文献类型：期刊论文

作者Zhang, Huan1,4; Wang, Hao1,4; Chen, Hao1,4; Wang, Mengqiang1,2; Zhou, Zhi2; Qiu, Limei1,2; Wang, Lingling3; Song, Linsheng3
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2019-05-01
卷号94页码:1-10
关键词Crassostrea gigas Transcriptome analysis Vibrio splendidus Immune challenge Heat stress Energy metabolic
ISSN号0145-305X
DOI10.1016/j.dci.2019.01.006
通讯作者Wang, Hao() ; Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要Bacterial infection and heat stress are considered as two major environmental threats for the aquaculture industry of oyster Crassostrea gigas. In the present study, the expression profiles of mRNA transcripts in the hemocytes of oysters under bacterial challenge and heat stress were examined by next-generation sequencing. There were 21,095, 21,957 and 21,141 transcripts identified in the hemocytes of oysters from three groups, respectively, including control group (designated as Con group), Vibrio splendidus challenge group (Bac group), and bacterial and heat stress combined treatment group (BacHeat group). There were 4610, 5093 and 5149 differentially expressed transcripts (DTs) in the three pairwise comparisons Con/Bac, Con/BacHeat and Bac/BacHeat, respectively. The main enriched GO terms in biological process category of the DTs included the metabolic processes, cellular process, response to stimulus and immune system process. The expression patterns of DTs involved in pattern recognition, immune signal transduction and energy metabolic indicated that the immune response to bacterial challenge was disturbed under acute heat stress, which was also confirmed by quantitative real-time PCR. The neuroendocrine immunomodulation, especially the catecholaminergic regulation, played indispensable roles in stress response. The total energy reserves as well as cellular energy allocation (CEA) in hepatopancreas of oysters decreased remarkably especially in BacHeat group, while the energy consumption generally increased, suggesting that the immune defense against the simultaneous stimulation of pathogen and heat stress imposed greater costs on oyster's energy expenditure than a single stressor. These results above indicated that, the heat stress disturbed the normal expression of genes involved in immune response and energy metabolism, accelerated energy consumption and broke the metabolic balance, leading to a decline in resilience to infection and mass mortality of oyster in summer.
资助项目National Science Foundation of China[41476145,31530069] ; [2018YFD0900606]
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
WOS记录号WOS:000460826100001
出版者ELSEVIER SCI LTD
源URL[http://ir.qdio.ac.cn/handle/337002/155244]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_深海极端环境与生命过程研究中心
通讯作者Wang, Hao; Song, Linsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, 52 Heishijiao St Dalian, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Huan,Wang, Hao,Chen, Hao,et al. The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,94:1-10.
APA Zhang, Huan.,Wang, Hao.,Chen, Hao.,Wang, Mengqiang.,Zhou, Zhi.,...&Song, Linsheng.(2019).The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,94,1-10.
MLA Zhang, Huan,et al."The transcriptional response of the Pacific oyster Crassostrea gigas under simultaneous bacterial and heat stresses".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 94(2019):1-10.

入库方式： OAI收割

来源：海洋研究所

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