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Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus

文献类型：期刊论文

作者Yuan, Jianbo1,2,3,4; Gao, Yi1,2,3,4; Sun, Lina1,2,3,4; Jin, Songjun1,2,3,4; Zhang, Xiaojun1,2,3,4; Liu, Chengzhang1,2,3,4; Li, Fuhua1,2,3,4; Xiang, Jianhai1,2,3,4
刊名FRONTIERS IN GENETICS
出版日期2019-02-19
卷号10页码:13
关键词sea cucumber intestinal regeneration Wnt signaling pathway positive selection natural selection
ISSN号1664-8021
DOI10.3389/fgene.2019.00112
通讯作者Yuan, Jianbo(yuanjb@qdio.ac.cn) ; Zhang, Xiaojun(xjzhang@qdio.ac.cn) ; Xiang, Jianhai(jhxiang@qdio.ac.cn)
英文摘要Many echinoderms are regenerative species that exhibit exceptional regenerative capacity, and sea cucumber is a representative organism that could regenerate the whole intestine after evisceration. There are many signaling pathways participate in the regeneration process, but it is not clear which is essential for the intestinal regeneration. In this study, we performed genome-wide comprehensive analyses on these regeneration-related signaling pathways, and found the Wnt signaling pathway was one of the most conservative pathways among regenerative species. Additionally, among these signaling pathways, we found that the Wnt signaling pathway was the only one under positive selection in regenerative echinoderms, and the only one enriched by differentially expressed genes during the intestinal regeneration. Thus, it suggests both coding sequence and gene expression of the Wnt signaling pathway have been shaped by natural selection to provide the genetic architecture for intestinal regeneration. Wnt7, Fz7, and Dvl are the three positively selected genes and also happen to be three upstream genes in the Wnt signaling pathway. They are all significantly upregulated at the early stages of regeneration, which may contribute significantly to the early activation of Wnt signaling and the initiation of intestinal regeneration. Expression knockdown of Wnt7 and Dvl by RNA interference significantly inhibit intestinal extension, implying that they are essential for intestinal regeneration. As an important regeneration-related gene, the downstream gene c-Myc is also conserved and highly expressed during the whole regeneration stages, which may make the Wnt/c-Myc signaling to be an important way to promote intestinal regeneration. Therefore, it is reasonable to conclude that the Wnt signaling pathway is the chosen one to play an important role in intestinal regeneration of sea cucumbers, or even in the regeneration of other echinoderms.
资助项目National Natural Science Foundation of China[41876167] ; National Natural Science Foundation of China[41776162] ; National Natural Science Foundation of China[41506189] ; China Agriculture Research system-47 (CARS-47) ; Senior User Project of RV KEXUE[KEXUE2018G19]
WOS研究方向Genetics & Heredity
语种英语
WOS记录号WOS:000459167300001
出版者FRONTIERS MEDIA SA
源URL[http://ir.qdio.ac.cn/handle/337002/155560]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Yuan, Jianbo; Zhang, Xiaojun; Xiang, Jianhai
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol & Marine Ecol & Envi, Qingdao, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Yuan, Jianbo,Gao, Yi,Sun, Lina,et al. Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN GENETICS,2019,10:13.
APA Yuan, Jianbo.,Gao, Yi.,Sun, Lina.,Jin, Songjun.,Zhang, Xiaojun.,...&Xiang, Jianhai.(2019).Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus.FRONTIERS IN GENETICS,10,13.
MLA Yuan, Jianbo,et al."Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus".FRONTIERS IN GENETICS 10(2019):13.

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来源：海洋研究所

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