Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection
文献类型：期刊论文
| 作者 | Zhang, Jian1,2,3; Sun, Li1,2 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2019-03-01 |
| 卷号 | 92页码:150-159 |
| 关键词 | Cynoglossus semilaevis Megalocytivirus Viral infection Immune defense Ubiquitin proteasome system |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2018.11.006 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Megalocytivirus is a DNA virus with a broad host range among farmed fish including tongue sole (Cynoglossus semilaevis). In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi). Compared to uninfected control fish, virus-infected fish displayed 315 up-regulated proteins and 111 down-regulated proteins at 8 dpi, and 48 up regulated proteins and 43 down-regulated proteins at 12 dpi. The expressions of five differentially expressed proteins were confirmed by Western blot. The differentially expressed proteins were enriched in the pathways and processes associated with innate immune response and viral infection. Interference with the expression of two up-regulated proteins of the ubiquitin proteasome system (UPS), i.e. proteasome assembly chaperone 2 and proteasome maturation protein, significantly reduced viral propagation in fish, whereas overexpression of these two proteins significantly enhanced viral propagation. Consistently, inhibition of the functioning of proteasome significantly impaired viral replication in vivo. This study provided the first global protein profile responsive to megalocytivirus in tongue sole, and revealed an essential role of UPS in viral infection. |
| 资助项目 | National Natural Science Foundation of China[31502197] ; National Natural Science Foundation of China[31730100] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000458596200016 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/155595]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Sun, Li. Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,92:150-159. |
| APA | Zhang, Jian,&Sun, Li.(2019).Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,92,150-159. |
| MLA | Zhang, Jian,et al."Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 92(2019):150-159. |
入库方式： OAI收割
来源：海洋研究所
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