Reference gene selection for microRNA expression analysis in the Pacific oyster
文献类型：期刊论文
| 作者 | Huang, Wen1,2,3,4 ; Xu, Fei1,3,4; Wang, Wei1,3,4; Li, Li1,2,4; Que, Huayong1,2,3,4; Zhang, Guofan1,2,3,4
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| 刊名 | ROMANIAN BIOTECHNOLOGICAL LETTERS
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| 出版日期 | 2018-09-01 |
| 卷号 | 23期号:5页码:14043-14050 |
| 关键词 | Crassostrea gigas MicroRNA Real-time quantitative PCR Reference gene |
| ISSN号 | 1224-5984 |
| DOI | 10.26327/RBL2018.224 |
| 英文摘要 | Real-time quantitative polymerase chain reaction (qRT-PCR) is a widely used technique for microRNA (miRNA) expression analysis. The accuracy and reproducibility of this technique depends on the availability of stably expressed reference genes, which is absent in molluscs. Here, based on the microRNA transcriptome data of the Pacific oyster, Crassostrea gigas, candidate reference genes were selected from the stably expressed microRNAs and the commonly used housekeeping genes (U1, U6, and 5.8S). The stability of expression was evaluated and the comprehensive ranking of the reference genes was obtained using two statistical algorithms, geNorm and NormFinder. Specific combinations of reference genes were recommended for the developmental larval samples (DLS) and adult organ samples (AOS), respectively; a combination of cgi-miR-71 and Ul was recommended for DLS and a combination of cgi-let-7, cgi-miR-87, and Ul for AOS, under normal physiological conditions. The effectiveness of the reference gene combinations was further validated by quantitative analysis of two selected miRNAs in the DLS and AOS, respectively. This work was the first systematic analysis of miRNA reference gene selection in molluscs. The reference genes identified in this study will be useful for the functional analysis of miRNA in Crassostrea gigas, and also for the miRNA reference gene selection in other molluscan species. |
| 资助项目 | National Natural Science Foundation of China[31372515] ; Guangxi Natural Science Foundation[2016GXNSFBA380028] ; China Agriculture Research System[CARS-49] ; Taishan Scholars Climbing Program of Shandong |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000454870500017 |
| 出版者 | ARS DOCENDI |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/155956]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Que, Huayong; Zhang, Guofan |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wen,Xu, Fei,Wang, Wei,et al. Reference gene selection for microRNA expression analysis in the Pacific oyster[J]. ROMANIAN BIOTECHNOLOGICAL LETTERS,2018,23(5):14043-14050. |
| APA | Huang, Wen,Xu, Fei,Wang, Wei,Li, Li,Que, Huayong,&Zhang, Guofan.(2018).Reference gene selection for microRNA expression analysis in the Pacific oyster.ROMANIAN BIOTECHNOLOGICAL LETTERS,23(5),14043-14050. |
| MLA | Huang, Wen,et al."Reference gene selection for microRNA expression analysis in the Pacific oyster".ROMANIAN BIOTECHNOLOGICAL LETTERS 23.5(2018):14043-14050. |
入库方式： OAI收割
来源：海洋研究所
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