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Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line

文献类型：期刊论文

作者Liu, Qianchun1,2,3; Wen, Changlong1; Zhao, Hong1; Wang, Yongqin1
刊名SCIENTIA HORTICULTURAE
出版日期2019-01-03
卷号243页码:101-106
关键词Welsh onion Cytoplasmic male sterility (CMS) atpA gene
ISSN号0304-4238
DOI10.1016/j.scienta.2018.08.020
通讯作者Wang, Yongqin(wangyongqin@nercv.org)
英文摘要Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen and is important for exploiting heterosis in crop plants, but the molecular mechanisms of CMS and fertility restoration remain poorly understood in Welsh onion. Some CMS-associated genes were previously identified by RNA-Seq as candidate genes mediating male sterility in Welsh onion. In this study, the ATPase isoenzyme and ATPase activity were investigated. We also performed 2-dimensional electrophoresis to screen for ATPase-related proteins. The ATP synthase subunit alpha was found to be specifically expressed in the CMS line. Furthermore, we found that the full length of the atpA coding sequence was the same length in the CMS line and its maintainer line, but single nucleotide polymorphisms (SNPs) that lead to amino acid substitutions in the translated proteins were found. We hypothesize that the atpA gene is involved in regulating the development of CMS.
资助项目National Natural Science Foundation of China[31372066] ; National Natural Science Foundation of China[31572119] ; Science and Technology Innovation Capability Construction of the Beijing Academy of Agriculture and Forestry Sciences[KJCX20170102]
WOS研究方向Agriculture
语种英语
WOS记录号WOS:000450381800013
出版者ELSEVIER SCIENCE BV
源URL[http://ir.qdio.ac.cn/handle/337002/156337]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Yongqin
作者单位1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Qianchun,Wen, Changlong,Zhao, Hong,et al. Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line[J]. SCIENTIA HORTICULTURAE,2019,243:101-106.
APA Liu, Qianchun,Wen, Changlong,Zhao, Hong,&Wang, Yongqin.(2019).Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line.SCIENTIA HORTICULTURAE,243,101-106.
MLA Liu, Qianchun,et al."Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line".SCIENTIA HORTICULTURAE 243(2019):101-106.

入库方式： OAI收割

来源：海洋研究所

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