Characteristics of Cyp11a during Gonad Differentiation of the Olive Flounder Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Liang, Dongdong1,2,3 ; Fan, Zhaofei1,2,3; Zou, Yuxia1,2,4; Tan, Xungang1,2,4; Wu, Zhihao1,2,4; Jiao, Shuang1,2,4; Li, Jun1,2,4; Zhang, Peijun1,2; You, Feng1,2,4
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2018-09-01 |
| 卷号 | 19期号:9页码:15 |
| ISSN号 | 1422-0067 |
| 关键词 | steroidogenic genes cloning expression regulation flatfish sex control |
| DOI | 10.3390/ijms19092641 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | The P450 side-chain cleavage enzyme, P450scc (Cyp11a) catalyzes the first enzymatic step for the synthesis of all steroid hormones in fish. To study its roles in gonads of the olive flounder Paralichthys olivaceus, an important maricultured fish species, we isolated the cyp11a genomic DNA sequence of 1396 bp, which consists of 5 exons and 4 introns. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) results indicated that the flounder cyp11a was exclusively expressed in gonad and head kidney tissues. Its expression level in the testis was higher than that in the ovary. According to the in situ hybridization patterns, cyp11a was mainly expressed in the Leydig cells of the testis, and the thecal cells of the ovary. Immunofluorescence analysis showed that Cyp11a was located in the cytoplasm of the cultured flounder testis cells. Further quantitative real-time PCR results presented the cyp11a differential expression patterns during gonad differentiation. Among different sampling points of the 17 beta-estradiol (E2, 5 ppm) treatment group, cyp11a expression levels were relatively high in the differentiating ovary (30 and 40mm total length, TL), and then significantly decreased in the differentiated ovary (80, 100 and 120 mm TL, p < 0.05). The pregnenolone level also dropped in the differentiated ovary. In the high temperature treatment group (HT group, 28 +/- 0.5 degrees C), the cyp11a expression level fluctuated remarkably in the differentiating testis (60 mm TL), and then decreased in the differentiated testis (80, 100 mm TL, p < 0.05). In the testosterone (T, 5 ppm) treatment group, the cyp11a was expressed highly in undifferentiated gonads and the differentiating testis, and then dropped in the differentiated testis. Moreover, the levels of cholesterol and pregnenolone of the differentiating testis in the HT and T groups increased. The expression level of cyp11a was significantly down-regulated after the cultured flounder testis cells were treated with 75 and 150 mu M cyclic adenosine monophosphate (cAMP), respectively (p < 0.05), and significantly up-regulated after treatment with 300 mu M cAMP (p < 0.05). Both nuclear receptors NR5a2 and NR0b1 could significantly up-regulate the cyp11a gene expression in a dosage dependent way in the testis cells detected by cell transfection analysis (p < 0.05). The above data provides evidence that cyp11a would be involved in the flounder gonad differentiation and development. |
| 资助项目 | National Natural Science Foundation of China[41276171] ; National Natural Science Foundation of China[31772834] ; National Flatfish Industry System Construction Program of China[nycytx-50] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000449988100177 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/156388]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 10049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Dongdong,Fan, Zhaofei,Zou, Yuxia,et al. Characteristics of Cyp11a during Gonad Differentiation of the Olive Flounder Paralichthys olivaceus[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(9):15. |
| APA | Liang, Dongdong.,Fan, Zhaofei.,Zou, Yuxia.,Tan, Xungang.,Wu, Zhihao.,...&You, Feng.(2018).Characteristics of Cyp11a during Gonad Differentiation of the Olive Flounder Paralichthys olivaceus.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(9),15. |
| MLA | Liang, Dongdong,et al."Characteristics of Cyp11a during Gonad Differentiation of the Olive Flounder Paralichthys olivaceus".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.9(2018):15. |
入库方式： OAI收割
来源：海洋研究所
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