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Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways

文献类型：期刊论文

作者Yang, Yue1,2,3,4; Xing, Ronge1,2,4; Liu, Song1,2,4; Qin, Yukun1,2,4; Li, Kecheng1,2,4; Yu, Huahua1,2,4; Li, Pengcheng1,2,4
刊名CARBOHYDRATE POLYMERS
出版日期2019-02-01
卷号205页码:401-409
关键词Hydroxypropyltrimethyl ammonium chloride chitosan Immunostimulatory activity ERK JNK P38 STAT
ISSN号0144-8617
DOI10.1016/j.carbpol.2018.10.101
通讯作者Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要Hydroxypropyltrimethyl ammonium chloride chitosan (HACC) is a water-soluble derivative of chitosan. To investigate the immunostimulatory effects of HACC, quaternized chitosans with different molecular weights were prepared and their effects on RAW 264.7 macrophages were compared. The results showed that HACC promoted nitric oxide (NO) production in a molecular weight- and dose-dependent manner. Lower molecular weight HACC was more active in promoting NO production. Furthermore, flow cytometry analysis showed that HACC significantly promoted the production of interleukin-6 and tumor necrosis factor-alpha. These results were further demonstrated by quantitive real-time reverse transcription polymerase chain reaction and western blot analysis. Moreover, western blotting revealed that HACC induced the phosphorylation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, and signal transducer and activator of transcription (STAT) proteins. In conclusion, HACC activated RAW 264.7 cells through the mitogen-activated protein kinases and Janus kinase/STAT pathways.
资助项目National Key R&D Program of China[2018YFC0311305] ; Special projects of Foshan science and technology innovation team[2017IT100054] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Key Research and Development Program of Shandong Province[2017YYSP018]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000450093200046
出版者ELSEVIER SCI LTD
源URL[http://ir.qdio.ac.cn/handle/337002/156391]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xing, Ronge; Li, Pengcheng
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Yue,Xing, Ronge,Liu, Song,et al. Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways[J]. CARBOHYDRATE POLYMERS,2019,205:401-409.
APA Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2019).Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways.CARBOHYDRATE POLYMERS,205,401-409.
MLA Yang, Yue,et al."Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways".CARBOHYDRATE POLYMERS 205(2019):401-409.

入库方式： OAI收割

来源：海洋研究所

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