High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)
文献类型：期刊论文
| 作者 | Wu Chunhui1,2,3 ; Jiang Peng1,2; Zhao Jin1,2; Fu Huihui1,2
|
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 36期号:5页码:1806-1811 |
| 关键词 | protoplast Uhva prolifera regeneration artificial culture |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-018-7058-0 |
| 通讯作者 | Jiang Peng(jiangpeng@qdio.ac.cn) |
| 英文摘要 | Protoplast isolation was relevant for gene manipulation in Ulva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transfomiation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. NeitherF(v)/F-m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an effective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast ((31.5 +/- 1.9)x 10(6) cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera. |
| 资助项目 | National Natural Science Foundation of China[41776153] ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02-1] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020304] ; Post-doctoral Application Research Program - Qingdao[2016189] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000450165300031 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/156409]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang Peng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu Chunhui,Jiang Peng,Zhao Jin,et al. High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(5):1806-1811. |
| APA | Wu Chunhui,Jiang Peng,Zhao Jin,&Fu Huihui.(2018).High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(5),1806-1811. |
| MLA | Wu Chunhui,et al."High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.5(2018):1806-1811. |
入库方式： OAI收割
来源：海洋研究所
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