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High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers

文献类型：期刊论文

作者Yi, Yunhai1,2; You, Xinxin2; Bian, Chao2; Chen, Shixi3,4; Lv, Zhao5; Qiu, Limei5; Shi, Qiong1,2,6
刊名MARINE DRUGS
出版日期2017-11-01
卷号15期号:11页码:18
关键词antimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity
ISSN号1660-3397
DOI10.3390/md15110364
通讯作者Shi, Qiong(shiqiong@genomics.cn)
英文摘要Widespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin 1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs.
资助项目Shenzhen Special Program for Development of Emerging Strategic Industries[JSGG20170412153411369] ; Zhenjiang Leading Talent Program for Innovation and Entrepreneurship
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000416600200034
出版者MDPI AG
源URL[http://ir.qdio.ac.cn/handle/337002/157299]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Shi, Qiong
作者单位1.Univ Chinese Acad Sci, IBGI Educ Ctr, Shenzhen 518083, Peoples R China
2.BGI, Shenzhen Key Lab Marine Gen, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Acad Marine Sci,BGI Marine, Shenzhen 518083, Peoples R China
3.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
4.Xiamen Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen 361102, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
6.Shenzhen Univ, Coll Life Sci & Oceanog, Lab Aquat Genom, Shenzhen 518060, Peoples R China
推荐引用方式
GB/T 7714		 Yi, Yunhai,You, Xinxin,Bian, Chao,et al. High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers[J]. MARINE DRUGS,2017,15(11):18.
APA Yi, Yunhai.,You, Xinxin.,Bian, Chao.,Chen, Shixi.,Lv, Zhao.,...&Shi, Qiong.(2017).High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers.MARINE DRUGS,15(11),18.
MLA Yi, Yunhai,et al."High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers".MARINE DRUGS 15.11(2017):18.

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来源：海洋研究所

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