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Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging

文献类型：期刊论文

作者Li, Tianmeng1,2,3; Hui, Hui2,3,4; Hu, Chaoen2,3; Ma, He1; Yang, Xin2,3; Tian, Jie2,3,4
刊名JOURNAL OF BIOMEDICAL OPTICS
出版日期2019
卷号24期号:1页码:8
关键词multimodality imaging confocal laser endomicroscopy light-sheet fluorescence microscopy magnetic resonance imaging inflammatory bowel disease
ISSN号1083-3668
DOI10.1117/1.JBO.24.1.016003
通讯作者Ma, He(mahe@bmie.neu.edu.cn) ; Yang, Xin(xin.yang@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
英文摘要The objective of our study is to develop a multimodality approach by combining magnetic resonance imaging (MRI) and optical imaging methods to assess acute murine colitis at the macro- and microscopic level. In vivo MRI is used to measure the cross-sectional areas of colons at the macroscopic level. Dual-color confocal laser endomicroscopy (CLE) allows in vivo examination of the fluorescently labeled epithelial cells and micro-vessels in the mucosa with a spatial resolution of similar to 1.4 mu m during ongoing endoscopy. To further validate the structural changes of the colons in three-dimensions, ex vivo light-sheet fluorescence microscopy (LSFM) is applied for in-toto imaging of cleared colon sections. MRI, LSFM, and CLE findings are significantly correlated with histological scoring (p < 0.01) and the inflammation-associated activity index (p < 0.01). Our multimodality imaging technique permits visualization of mucosa in colitis at different scales, which can enhance our understanding of the pathogenesis of inflammatory bowel diseases. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
WOS关键词INFLAMMATORY-BOWEL-DISEASE ; MOUSE MODEL ; COLONOSCOPY ; MUCOSA ; CANCER
资助项目National Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81671851] ; National Natural Science Foundation of China[81227901] ; Strategic Priority Research Program[XDBS01030200] ; Scientific Instrument RD Programs[GJJSTD20170004] ; Scientific Instrument RD Programs[YJKYYQ20170075] ; Frontier Science Key Research Program from the Chinese Academy of Sciences[QYZDJ-SSW-JSC005] ; Beijing Municipal Science and Technology Commission[Z161100002616022]
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种英语
WOS记录号WOS:000463884000008
出版者SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Scientific Instrument RD Programs ; Frontier Science Key Research Program from the Chinese Academy of Sciences ; Beijing Municipal Science and Technology Commission
源URL[http://ir.ia.ac.cn/handle/173211/24928]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Ma, He; Yang, Xin; Tian, Jie
作者单位1.Northeastern Univ, Sino Dutch Biomed & Informat Engn Sch, Shenyang, Liaoning, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing, Peoples R China
3.Inst Automat, Beijing Key Lab Mol Imaging, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Tianmeng,Hui, Hui,Hu, Chaoen,et al. Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging[J]. JOURNAL OF BIOMEDICAL OPTICS,2019,24(1):8.
APA Li, Tianmeng,Hui, Hui,Hu, Chaoen,Ma, He,Yang, Xin,&Tian, Jie.(2019).Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging.JOURNAL OF BIOMEDICAL OPTICS,24(1),8.
MLA Li, Tianmeng,et al."Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging".JOURNAL OF BIOMEDICAL OPTICS 24.1(2019):8.

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来源：自动化研究所

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