Recently, segmentectomy has become a secure and effective treatment for certain small, early-stage lung cancer, especially in patients with emphysema. To achieve complete segmentectomy, it is critical to precisely identify adjacent lung segments, which is difficult for surgeons without suitable interventions. Thus, a novel technique by transbronchial or intravenous injection of indocyanine green (ICG) has been developed, which can efficiently avert needless resection, lower the costs and reduce complications. However, lack of intraoperative fluorescence imaging systems has seriously impeded the further development of this method. So, we developed a novel wireless wearable fluorescence surgical navigation system (WFNS), which combined the mobility of the goggle system and the sensitivity of other imaging systems. To test the mobility and sensitivity of WFNS, twelve in vivo studies of pulmonary segmentectomy in swine by intravenous injection of ICG were executed.