A novel, enzyme-linked immunosorbent assay based on the catalysis of AuNCs@BSA-induced signal amplification for the detection of dibutyl phthalate
文献类型：期刊论文
| 作者 | Zhang, Zhen; Zhu, Nuanfei; Zou, Yanmin; Wu, Xiangyang; Qu, Guangbo; Shi, Jianbo |
| 刊名 | TALANTA
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 179页码:64-69 |
| 关键词 | Enzyme-linked Immunosorbent Assay (ELISA) Immunoassay AuNCs AgNPs Dibutyl Phthalate (DBP) |
| ISSN号 | 0039-9140 |
| 文献子类 | Article |
| 英文摘要 | A novel, competitive, enzyme-linked immunosorbent assay (ELISA) was presented in this paper based on the inhibition of catalysis of AuNCs@BSA triggered by dissolved Ag+ for the detection of dibutyl phthalate (DBP). In the immunoassay system, numerous Ag+ was released from AgNPs (labelled on the second antibody, AgNPs@Ab(2)) in the presence of H2O2 after the competition step, preventing AuNCs@BSA from inducing a color change of 3,3',5,5'-tetramethylbenzidine (TMB) to blue. Due to the signal amplification by the principle, the sensitivity of the modified ELISA was improved with the low limit of detection (LOD) of 4.017 mu g/L for DBP, which was decreased 16 times relative to that using conventional ELISA with the same antibody. In addition, the established method showed satisfactory accuracy and reliability (recoveries, 85.75-117.73%; CV, 1.33-6.79%) in spike-recovery analysis. To the best of our knowledge, this is the first time that AuNCs@BSA has been used in ELISA as a peroxidase-like catalyst. Our method shows great potential for trace DBP detection from environmental and food samples. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40864]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Zhen,Zhu, Nuanfei,Zou, Yanmin,et al. A novel, enzyme-linked immunosorbent assay based on the catalysis of AuNCs@BSA-induced signal amplification for the detection of dibutyl phthalate[J]. TALANTA,2018,179:64-69. |
| APA | Zhang, Zhen,Zhu, Nuanfei,Zou, Yanmin,Wu, Xiangyang,Qu, Guangbo,&Shi, Jianbo.(2018).A novel, enzyme-linked immunosorbent assay based on the catalysis of AuNCs@BSA-induced signal amplification for the detection of dibutyl phthalate.TALANTA,179,64-69. |
| MLA | Zhang, Zhen,et al."A novel, enzyme-linked immunosorbent assay based on the catalysis of AuNCs@BSA-induced signal amplification for the detection of dibutyl phthalate".TALANTA 179(2018):64-69. |
入库方式： OAI收割
来源：生态环境研究中心
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