中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach

文献类型:期刊论文

作者Yan, Xueting; He, Bin; Liu, Lihong; Qu, Guangbo; Shi, Jianbo; Hu, Ligang; Jiang, Guibin
刊名METALLOMICS
出版日期2018-04-01
卷号10期号:4页码:557-564
ISSN号1756-5901
文献子类Article
英文摘要Silver nanoparticles (AgNPs) are the nanomaterials most widely used as antimicrobial agents in a range of consumer products, due to the environmental release of either the AgNPs themselves or silver ions. Although AgNPs appear to be more potent than silver ions, the mechanism behind the activity is not fully elucidated yet. The most common mechanism of toxicity of AgNPs proposed to date is the release of silver ions and/or the particle-specific functions. In this study, Pseudomonas aeruginosa (a model for Gram-negative bacteria) was treated with AgNPs, and its proteomic response was comprehensively characterized to elucidate the antimicrobial mechanism of AgNPs in the microorganism. In total, 59 silver-regulated proteins (27 up-regulated and 32 down-regulated proteins) and 5 silver-binding proteins were identified. Bioinformatic analysis revealed that interference with the cell-membrane function and generation of intracellular reactive oxygen species (ROS) were the main pathways for the antibacterial effect. The pattern of membrane proteins regulated by AgNPs was similar to that found for silver ions. In addition, the same silver-binding proteins were obtained with both AgNPs and silver ions, which indicated that AgNPs probably affect the cell membrane and react with proteins by releasing silver ions. The elevation of intracellular ROS relative to that with silver ions confirmed oxidative damage caused by AgNPs, which may be ascribed to the nano-characteristics and higher uptake efficiency of the particles. These results demonstrate that the antimicrobial activity of AgNPs is due to the synergistic action of release of dissolved silver ions and particle-specific effects. The proteomic analysis of silver-binding and silver-regulated proteins in the present study provides insight into the mechanism of antimicrobial activity of such nanomaterials.
源URL[http://ir.rcees.ac.cn/handle/311016/40877]  
专题生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式
GB/T 7714
Yan, Xueting,He, Bin,Liu, Lihong,et al. Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach[J]. METALLOMICS,2018,10(4):557-564.
APA Yan, Xueting.,He, Bin.,Liu, Lihong.,Qu, Guangbo.,Shi, Jianbo.,...&Jiang, Guibin.(2018).Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach.METALLOMICS,10(4),557-564.
MLA Yan, Xueting,et al."Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach".METALLOMICS 10.4(2018):557-564.

入库方式: OAI收割

来源:生态环境研究中心

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。