Characterization of calmodulin in the clam Anodonta woodiana: differential expressions in response to environmental Ca2+ and Cd2+
文献类型：期刊论文
| 作者 | Xia, Xichao; Liang, Guina; Zheng, Xinhua; Wang, Fuan; Zhang, Junfeng; Xue, Shipeng; Hua, Chuanxiu; Song, Guoying; Bai, Xianguang; Guo, Lianghong |
| 刊名 | TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 43期号:4页码:403-416 |
| 关键词 | Anodonta woodiana AwCaM1 Up-regulations Ca2+ Cd2+ |
| ISSN号 | 0250-4685 |
| 文献子类 | Article |
| 英文摘要 | Aims: To explore effect of Ca2+ and Cd2+ on the calmodulin (CaM), one complete cDNA sequence (AwCaM1) was cloned and characterized from the freshwater mussel Anodonta woodiana and its expressions were analyzed. Materials and methods: The AwCaM1 was cloned from the A. woodiana using the rapid amplification of cDNA ends methods and its expression was determined by real-time PCR. Results: In the hepatopancreas, AwCaM1 expression was up-regulated with a time and dose dependent pattern in the Ca2+ treated groups (0.01, 0.02, 0.04 and 0.08 mg/L) during experiment observed, and increased more than 56.15% (p < 0.05) compared with that of control group. AwCaM1 mRNA level increased more 65.04% (p < 0.05) in the Cd2+ treated groups (8 and 16 mg/L). In the gill, AwCaM1 expression increased more than 79.41% (p < 0.05) compared with that of control group in all the Ca2+ treated groups, and more than 88.23% (p < 0.05) in all the Cd2+ treated groups. Conclusion: These results indicated that up-regulations of AwCaM1 expression in bivalve A. woodiana are associated with Ca2+ absorb and environmental adaption derived from Ca2+ and Ca2+ treatment. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40896]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xia, Xichao,Liang, Guina,Zheng, Xinhua,et al. Characterization of calmodulin in the clam Anodonta woodiana: differential expressions in response to environmental Ca2+ and Cd2+[J]. TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI,2018,43(4):403-416. |
| APA | Xia, Xichao.,Liang, Guina.,Zheng, Xinhua.,Wang, Fuan.,Zhang, Junfeng.,...&Guo, Lianghong.(2018).Characterization of calmodulin in the clam Anodonta woodiana: differential expressions in response to environmental Ca2+ and Cd2+.TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI,43(4),403-416. |
| MLA | Xia, Xichao,et al."Characterization of calmodulin in the clam Anodonta woodiana: differential expressions in response to environmental Ca2+ and Cd2+".TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI 43.4(2018):403-416. |
入库方式： OAI收割
来源：生态环境研究中心
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