Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography
文献类型：期刊论文
| 作者 | Li, Yiling; He, Bin; Hu, Ligang; Huang, Xiu; Yun, Zhaojun; Liu, Runzeng; Zhou, Qunfang; Jiang, Guibin
|
| 刊名 | TALANTA
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 178页码:811-817 |
| 关键词 | Immobilized mercury ion affinity chromatography Mercury Proteins SK-N-SH cell |
| ISSN号 | 0039-9140 |
| 文献子类 | Article |
| 英文摘要 | Metal-binding proteins play important roles in biological functions of metals. However, only very limited mercury-binding proteins with high abundance were characterized in cells or organisms. Characterization of mercury-binding proteins in proteome-wide is important for elucidating mechanisms of mercury toxicity comprehensively. In this study, a method based on immobilized mercury ion affinity chromatography was developed for identification of putative mercury-binding proteins. The method was then successfully applied to profile mercury-binding proteins in human neuroblastoma SK-N-SH cells. In total; 38 proteins were identified as mercury-binding proteins, in which most of them were uncharacterized to associate with mercury in cells. The identified mercury-binding proteins did not show obvious relevance to protein abundance and were mainly involved in protein processing in endoplasmic reticulum, protein folding, and cytoskeleton organization. The newly built metalloproteomic approach provided valuable information on the possible molecular mechanisms and protein candidates for mercury transport and toxicity. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40897]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Yiling,He, Bin,Hu, Ligang,et al. Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography[J]. TALANTA,2018,178:811-817. |
| APA | Li, Yiling.,He, Bin.,Hu, Ligang.,Huang, Xiu.,Yun, Zhaojun.,...&Jiang, Guibin.(2018).Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography.TALANTA,178,811-817. |
| MLA | Li, Yiling,et al."Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography".TALANTA 178(2018):811-817. |
入库方式： OAI收割
来源：生态环境研究中心
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