Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of Aflatoxin B1
文献类型：期刊论文
| 作者 | Sun, Linlin; Zhao, Qiang |
| 刊名 | TALANTA
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 179页码:344-349 |
| 关键词 | Aptamer Aflatoxin B1 Competitive assay Enzyme label Absorbance analysis Chemiluminescence analysis |
| ISSN号 | 0039-9140 |
| 文献子类 | Article |
| 英文摘要 | Aflatoxin B1 (AFB1) is one of highly toxic mycotoxins and a known human carcinogen. The frequent contamination of AFB1 in food products and large health risk of AFB1 have raised global concerns. Sensitive detection of AFB1 is of vital importance and highly demanded. Herein, we reported a competitive horseradish peroxidase (HRP)-linked aptamer assay for AFB1, combining the advantages of aptamer for affinity binding and enzyme label for signal amplification. In this assay, free AFB1 in solution competed with a covalent conjugate of bovine serum albumin-AFB1 (BSA-AFB1) coated on the wells of microplate in binding to the HRP-labeled aptamer probe. HRP attached on BSA-AFB1 in the wells catalyzed the conversion of substrates into products, allowing the final detection of AFB1 through measurement of the generated products. When TMB (3,3',5,5'-tetramethylbenzidine dihydrochloride) was used as substrate, absorbance analysis of the product of enzyme reaction enabled the detection of AFB1 at 0.2 nM. We further lowered the detection limit of AFB1 to 0.01 nM through chemiluminescence analysis by using chemiluminescence substrate of HRP. This assay enabled the detection of AFB1 in complex sample matrix, such as diluted white wine and maize flour. This assay provides a simple, sensitive and rapid method for AFB1 determination. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40904]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Linlin,Zhao, Qiang. Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of Aflatoxin B1[J]. TALANTA,2018,179:344-349. |
| APA | Sun, Linlin,&Zhao, Qiang.(2018).Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of Aflatoxin B1.TALANTA,179,344-349. |
| MLA | Sun, Linlin,et al."Competitive horseradish peroxidase-linked aptamer assay for sensitive detection of Aflatoxin B1".TALANTA 179(2018):344-349. |
入库方式： OAI收割
来源：生态环境研究中心
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