Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples
文献类型：期刊论文
| 作者 | Zhou, Zhiguang; Zhang, Songyan; Xu, Li; Fu, Hualing; Li, Shuaizhang; Zhao, Bin; Xie, Heidi Qunhui |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2018-03-06 |
| 卷号 | 52期号:5页码:2926-2933 |
| ISSN号 | 0013-936X |
| 文献子类 | Article |
| 英文摘要 | Airborne persistent toxic substances are associated with health impacts resulting from air pollution, for example, dioxins, dioxin like polychlorinated biphenyls, and certain polycyclic aromatic hydrocarbons (PAHs), which activate aryl hydrocarbon receptors (AhR) and thereby produce adverse outcomes. Thus, a bioassay for evaluating AhR activation is required for risk assessment of ambient-air samples, and for this purpose, we developed a new and sensitive recombinant mouse hepatoma cell line, CBG2.8D, in which a novel luciferase-reporter plasmid containing two copies of a newly designed dioxin-responsive domain and a minimal promoter derived from a native gene were integrated. The minimal detection limit for 2,3,7,8-tetrachlorodibenzo-p-dioxin with this assay system was 0.1 pM. We used CBG2.8D to determine dioxin levels in 45 ambient-air samples collected in Beijing. The measured bioanalytical equivalent (BEQ) values were closely correlated with the toxic equivalent values obtained from chemical analysis. In haze ambient-air samples, the total activation of aryl hydrocarbon receptors (TAA) was considerably higher than the BEQof dioxin-rich fractions, according to the results of the cell-based bioassay. Notably, the haze samples contained abundant amounts of PAHs, whose relative toxicity equivalent was correlated with the TAA; this finding suggests that PAHs critically contribute to the AhR-related biological impacts of haze ambient-air samples. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40916]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhou, Zhiguang,Zhang, Songyan,Xu, Li,et al. Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2018,52(5):2926-2933. |
| APA | Zhou, Zhiguang.,Zhang, Songyan.,Xu, Li.,Fu, Hualing.,Li, Shuaizhang.,...&Xie, Heidi Qunhui.(2018).Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples.ENVIRONMENTAL SCIENCE & TECHNOLOGY,52(5),2926-2933. |
| MLA | Zhou, Zhiguang,et al."Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples".ENVIRONMENTAL SCIENCE & TECHNOLOGY 52.5(2018):2926-2933. |
入库方式： OAI收割
来源：生态环境研究中心
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