Gonadal differentiation and its sensitivity to androgens during development of Pelophylax nigromaculatus
文献类型：期刊论文
| 作者 | Li, Yuan-Yuan; Meng, Tan; Gao, Kun; Qin, Zhan-Fen |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 202页码:188-195 |
| 关键词 | Pelophylax nigromaculatus Gonadal differentiation Endocrine disrupting chemicals Masculinizing effects Sensitive stage |
| ISSN号 | 0166-445X |
| 文献子类 | Article |
| 英文摘要 | Our previous observations proposed Pelophylax nigromaculatus as a model species for studying the masculinizing effects of androgenic EDCs in amphibians. To better develop this model species, we studied the process of the gonadal differentiation/development and the sensitive stage to androgens. We found that the earliest sexual dimorphism in gonads at morphological and histological levels occurred at stages 38-40 and stage 36 respectively. Further examination of molecular markers for testicular and ovarian differentiation during development revealed that the cyp17 and cyp19 expressions were sexually dimorphic from stage 32 and stage 36 respectively. Further, we investigated the sex-reversal induced by 100 ng/L 5a-dihydrotestosterone (DHT) when exposures were initiated at stages 24, 26 and 28. We found that when exposed from stage 24, DHT resulted masculinization of all tadpoles with no typical ovaries, whereas exposures from stage 26 or 28 dramatically reduced the effect of DHT. Our findings show that gonads of P. nigromaculatus are bipotential at stage 24, in the process of differentiation at stage 26 and determined to become either testis or ovary at stage 28. Altogether, exposure of P. nigromaculatus should begin at stage 24 in order to sensitively detect masculinizing effects of EDCs. Present study provides useful information about the gonadal differentiation and development in P. nigromaculatus for effectively evaluating masculinizing effects of EDCs on gonads. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40950]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Yuan-Yuan,Meng, Tan,Gao, Kun,et al. Gonadal differentiation and its sensitivity to androgens during development of Pelophylax nigromaculatus[J]. AQUATIC TOXICOLOGY,2018,202:188-195. |
| APA | Li, Yuan-Yuan,Meng, Tan,Gao, Kun,&Qin, Zhan-Fen.(2018).Gonadal differentiation and its sensitivity to androgens during development of Pelophylax nigromaculatus.AQUATIC TOXICOLOGY,202,188-195. |
| MLA | Li, Yuan-Yuan,et al."Gonadal differentiation and its sensitivity to androgens during development of Pelophylax nigromaculatus".AQUATIC TOXICOLOGY 202(2018):188-195. |
入库方式： OAI收割
来源：生态环境研究中心
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