Insight into the endocrine disrupting effect and cell response to butyltin compounds in H295R cell: Evaluated with proteomics and bioinformatics analysis
文献类型：期刊论文
| 作者 | Yan, Xueting; He, Bin; Hu, Ligang; Gao, Jiejun; Chen, Shuai; Jiang, Guibin
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 628-629页码:1489-1496 |
| 关键词 | Butyltin compounds H295R cell Endocrine disruption Proteotnics Bioinformatics analysis |
| ISSN号 | 0048-9697 |
| 文献子类 | Article |
| 英文摘要 | The widespread use of organotin compounds (OTs) as biocides in antifouling paints and agricultural applications poses a serious threat to the ecosystem and humans. Butyltin compounds (BTs), especially tributyltin (TBT), are considered to be endocrine disrupting chemicals in marine organisms. The underlying mechanism of disrupting effects on mammals, however, has not been sufficiently investigated. To determine the effect and action of these biocides, the present study evaluated the effects of BTs on human adrenocortical carcinoma cells (H295R) with a focus on endocrine disrupting effect. Two-dimensional electrophoresis (2-DE) and subsequent mass finger printing were used to identify proteins expression profiles from the cells after exposure to 0.1 mu M BTs for 48 h. In total, 89 protein spots showed altered expression in at least two treatment groups and 69 of these proteins were subsequently identified. Bioinformatic analysis of the proteins indicated that BTs involved in the regulation of hormone homeostasis, lipid metabolism, cell death, and energy production. IPA analysis revealed IXR/RXR (liver X receptor/retinoid X receptor) activation, EXR/RXR (famesoid X receptor/retinoid X receptor) activation and fatty add metabolism were the top three categories on which BTs acted and these systems play vital roles in sterol, glucose and lipid metabolism. The expression of LXR and FXR mRNA in H295R cells was stimulated by TBT, confirming the ability of TBT to activate this nuclear receptor. In summary, the differentially expressed proteins discovered in this study may participate in the toxic actions of BTs, and nuclear receptor activation and lipid metabolism may play important roles in such actions of BTs. (C) 2018 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40970]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yan, Xueting,He, Bin,Hu, Ligang,et al. Insight into the endocrine disrupting effect and cell response to butyltin compounds in H295R cell: Evaluated with proteomics and bioinformatics analysis[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2018,628-629:1489-1496. |
| APA | Yan, Xueting,He, Bin,Hu, Ligang,Gao, Jiejun,Chen, Shuai,&Jiang, Guibin.(2018).Insight into the endocrine disrupting effect and cell response to butyltin compounds in H295R cell: Evaluated with proteomics and bioinformatics analysis.SCIENCE OF THE TOTAL ENVIRONMENT,628-629,1489-1496. |
| MLA | Yan, Xueting,et al."Insight into the endocrine disrupting effect and cell response to butyltin compounds in H295R cell: Evaluated with proteomics and bioinformatics analysis".SCIENCE OF THE TOTAL ENVIRONMENT 628-629(2018):1489-1496. |
入库方式： OAI收割
来源：生态环境研究中心
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