Investigation of the Mechanism of Cellular Uptake, Distribution and Toxicity of the DNA 'Light-Switch' Ru(II) Polypyridyl Complexes
文献类型：期刊论文
| 作者 | Tang, Miao; Zhu, Benzhan; Xiao, Xuan; Chao, Xijuan; Huang, Rong; Shao, Jie |
| 刊名 | PROGRESS IN CHEMISTRY
 |
| 出版日期 | 2018-12-15 |
| 卷号 | 30期号:12页码:1975-1991 |
| ISSN号 | 1005-281X |
| 关键词 | Ru (II) polypyridyl complexes pentachlorophenol (PCP) nuclear uptake ion-pairing luminescent DNA probes |
| 文献子类 | Review |
| 英文摘要 | Since the discovery of DNA as the genetic material carrier, the work towards the elucidation of DNA structure within the cell nucleus has become of great importance. Fluorescent microscopy using luminescent and cell membrane permeable organic DNA-binding molecule as probes is a well-established technique towards achieving this goal. Barton' s group discovered that the cationic ruthenium complex [Ru (bpy)(2) (dppz)](2+) (bpy = 2,2 '-bipyridine, dppz = dipyrido[3,2-a;2',3'-c]phenazine) functions as a molecular ' light switch' for DNA. Since then, there has been great attention drawn to the DNA binding properties of polypyridyl complexes of d(6) octahedral metal ions, specifically towards the development of highly sensitive and structure-specific DNA probes. Until recently the research has been largely focused on the development of in vitro probes. However, few studies involving direct imaging of DNA in live cells with such systems have had very limited success, with poor membrane permeability still being ascribed as the major limiting factor. We found that not only the cellular, but more interestingly and importantly, the nuclear uptake of [Ru (bpy)(2) (dppz)](2+) is remarkably enhanced by pentachlorophenol and two other structurally unrelated biochemical agents. Furthermore, enantioselective imaging of live-cell nuclear DNA is observed between the two chiral forms of Ru (II) complexes. The underlying molecular mechanism is found to be the formation of novel lipophilic and relatively stable ion-pair complexes. This represents the first report for an unprecedented new method for delivering the DNA light-switching' Ru (II) complexes into the nucleus of living cells via ion-pairing, which could serve as a promising general live-cell delivering method for other potentially bio-medically but cell-impermeable metal complexes. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40973]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Tang, Miao,Zhu, Benzhan,Xiao, Xuan,et al. Investigation of the Mechanism of Cellular Uptake, Distribution and Toxicity of the DNA 'Light-Switch' Ru(II) Polypyridyl Complexes[J]. PROGRESS IN CHEMISTRY,2018,30(12):1975-1991. |
| APA | Tang, Miao,Zhu, Benzhan,Xiao, Xuan,Chao, Xijuan,Huang, Rong,&Shao, Jie.(2018).Investigation of the Mechanism of Cellular Uptake, Distribution and Toxicity of the DNA 'Light-Switch' Ru(II) Polypyridyl Complexes.PROGRESS IN CHEMISTRY,30(12),1975-1991. |
| MLA | Tang, Miao,et al."Investigation of the Mechanism of Cellular Uptake, Distribution and Toxicity of the DNA 'Light-Switch' Ru(II) Polypyridyl Complexes".PROGRESS IN CHEMISTRY 30.12(2018):1975-1991. |
入库方式： OAI收割
来源：生态环境研究中心
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