MALDI-MS Imaging Reveals Asymmetric Spatial Distribution of Lipid Metabolites from Bisphenol S-Induced Nephrotoxicity
文献类型：期刊论文
| 作者 | Cai, Zongwei; Zhao, Chao; Xie, Peisi; Yong, Ting; Wang, Hailin; Chung, Arthur Chi Kong |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-03-06 |
| 卷号 | 90期号:5页码:3196-3204 |
| ISSN号 | 0003-2700 |
| 文献子类 | Article |
| 英文摘要 | With the continuous exposure of environmental pollutants in organisms, determination of abundance variation and spatial distribution of lipids might expand our understanding of toxicological mechanisms occurring in the kidney. Herein, an integrated method involving mass spectrometry (MS)-based lipidomics and matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) was developed for the study of nephrotoxicity in mice exposed to 10 and 100 pg bisphenol S (BPS)/kg body weight/day. The BPS exposure remarkable perturbed abundances of 91 potential markers that mainly involved in five metabolic pathways. We elucidated the lipids spatial heterogeneity by using morphological analysis, probabilistic latent semantic analysis, and coregistered multimodal three-dimensional (3D)-MSI. In morphological analysis, both 10 and 100 mu g BPS induced significant nephrotoxicity to mice, including glomerular necrosis in renal cortex, cloudy swelling in renal medulla, and interstitial collapsing in renal pelvis. Significant differential signaling lipids such as sphingomyelin (SM) (d22:0/20:4), ceramide (Cer) (d18:2/24:1), and sphingosine (d18:0) related to inflammation were found to be up-regulated and colocalized in the renal cortex, medulla, and pelvis, respectively. Also, seven significant differential lipids, which are considered to be involved in membrane homeostasis and cellular function, were found to be colocalized in the renal cortex. The observed significant variations of morphology, lipid accumulation, and metabolism in the renal cortex implicated that lipids in the renal cortex were more sensitive to BPS exposure than those in the renal medulla and pelvis. Moreover, we reconstructed a 3D-MSI model of kidney and identified two heterogeneous-related substructures in the renal cortex and pelvis upon 100 mu g BPS exposure. It might be used in novel specificity evaluation and early diagnosis for environmental pollutant-induced kidney diseases. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40978]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Cai, Zongwei,Zhao, Chao,Xie, Peisi,et al. MALDI-MS Imaging Reveals Asymmetric Spatial Distribution of Lipid Metabolites from Bisphenol S-Induced Nephrotoxicity[J]. ANALYTICAL CHEMISTRY,2018,90(5):3196-3204. |
| APA | Cai, Zongwei,Zhao, Chao,Xie, Peisi,Yong, Ting,Wang, Hailin,&Chung, Arthur Chi Kong.(2018).MALDI-MS Imaging Reveals Asymmetric Spatial Distribution of Lipid Metabolites from Bisphenol S-Induced Nephrotoxicity.ANALYTICAL CHEMISTRY,90(5),3196-3204. |
| MLA | Cai, Zongwei,et al."MALDI-MS Imaging Reveals Asymmetric Spatial Distribution of Lipid Metabolites from Bisphenol S-Induced Nephrotoxicity".ANALYTICAL CHEMISTRY 90.5(2018):3196-3204. |
入库方式： OAI收割
来源：生态环境研究中心
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