Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional
文献类型：期刊论文
| 作者 | Qu, Guangbo; Li, Yiling; He, Bin; Gao, Jiejun; Liu, Qian S.; Liu, Runzeng; Shi, Jianbo; Hu, Ligang; Jiang, Guibin
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| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 238页码:213-221 |
| ISSN号 | 0269-7491 |
| 关键词 | Methylmercury Mercury chloride Differentially expressed proteins Alternative splicing SK-N-SH cells |
| 文献子类 | Article |
| 英文摘要 | The neurotoxic effects of methylmercury (MeHg) have been intensively studied. However, the molecular mechanisms responsible for the neurotoxicity of MeHg are not fully understood. To decipher these mechanisms, proteomic and high-throughput mRNA sequencing (RNA-seq) technique were utilized, comprehensively evaluating the cellular responses of human neuroblastoma SK-N-SH cells to MeHg exposure. Proteomic results revealed that MeHg exposure interfered with RNA splicing via splicesome, along with the known molecular mechanisms of mercury-related neurotoxicity (e.g. oxidative stress, protein folding, immune system processes, and cytoskeletal organization). The effects of MeHg on RNA splicing were further verified using RNA-seq. Compared to control, a total of 658 aberrant RNA alternative splicing (AS) events were observed after MeHg exposure. Proteomics and RNA-seq results also demonstrated that mercury chloride (HgCl2) influenced the expression levels of several RNA splicing related proteins and 676 AS events compared to control. These results suggested that RNA splicing could be a new molecular mechanism involved in MeHg and HgCl2 neurotoxicity. (C) 2018 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40982]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Qu, Guangbo,Li, Yiling,He, Bin,et al. Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional[J]. ENVIRONMENTAL POLLUTION,2018,238:213-221. |
| APA | Qu, Guangbo.,Li, Yiling.,He, Bin.,Gao, Jiejun.,Liu, Qian S..,...&Jiang, Guibin.(2018).Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional.ENVIRONMENTAL POLLUTION,238,213-221. |
| MLA | Qu, Guangbo,et al."Methylmercury exposure alters RNA splicing in human neuroblastoma SK-N-SH cells: Implications from proteomic and post-transcriptional".ENVIRONMENTAL POLLUTION 238(2018):213-221. |
入库方式： OAI收割
来源：生态环境研究中心
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