Multihierarchically Profiling the Biological Effects of Various Metal-Based Nanoparticles in Macrophages under Low Exposure Doses
文献类型：期刊论文
| 作者 | Wang, Shunhao; Li, Ruibin; Zhang, Jie; Liu, Sijin; Gao, Ming |
| 刊名 | ACS SUSTAINABLE CHEMISTRY & ENGINEERING
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 6期号:8页码:10374-10384 |
| 关键词 | Metal-based nanoparticles Macrophages Multihierarchic profiles Adverse outcome pathways Low-dose exposure |
| ISSN号 | 2168-0485 |
| 文献子类 | Article |
| 英文摘要 | Thus far, tremendous efforts have been made to understand the biosafety of metal-based nanoparticles (MNPs). Nevertheless, most previous studies focused on specific adverse outcomes of MNPs at unrealistically high concentrations with little relevance to the National Institute for Occupational Safety and Health (NIOSH) exposure thresholds, and failed to comprehensively evaluate their toxicity profiles. To address these challenges, we here endeavored to multihierarchically profile the hazard effects of various popularly used MNPs in macrophages under low exposure doses. At these doses, no remarkable cell viability drop and cell death were induced. However, a cellular antioxidant defense system was seen to be initiated in cells by all MNPs even at these low concentrations, albeit to a differential extent and through different pathways, as reflected by differential induction of the antioxidant enzymes and Nrf2 signaling. Regarding inflammation, rare earth oxide nanomaterials (REOs) except nCeO(2) greatly increased IL-1 beta secretion in a NLRP3 inflammasome-dependent manner. By contrast, six REOs, AgNP-Snm, nFe(2)O(3), nFe(3)O(4), and nZnO were found to elevate TNF-alpha concentration through post-transcriptional regulation. Moreover, all MNPs except nCeO(2) drastically altered cellular membrane/cytoskeleton meshwork, but leading to different outcomes, with condensed cellular size and reduced numbers of protrusions by REOs and elongated protrusions by other MNPs. Consequently, REOs (e.g., nDy(2)O(3) and nSm(2)O(3)) impaired phagocytosis of macrophages, and other MNPs (such as AgNP-25nm and nZnO) reversely enhanced macrophagic phagocytosis. Alterations of membrane and cytoskeleton meshwork induced by these MNPs also caused disordered membrane potential and calcium ion flux. Collectively, our data profiled the biological effects of different MNPs in macrophages under low exposure doses, and deciphered a complex network that links multiparallel pathways and processes to differential adverse outcomes. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40993]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Shunhao,Li, Ruibin,Zhang, Jie,et al. Multihierarchically Profiling the Biological Effects of Various Metal-Based Nanoparticles in Macrophages under Low Exposure Doses[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2018,6(8):10374-10384. |
| APA | Wang, Shunhao,Li, Ruibin,Zhang, Jie,Liu, Sijin,&Gao, Ming.(2018).Multihierarchically Profiling the Biological Effects of Various Metal-Based Nanoparticles in Macrophages under Low Exposure Doses.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,6(8),10374-10384. |
| MLA | Wang, Shunhao,et al."Multihierarchically Profiling the Biological Effects of Various Metal-Based Nanoparticles in Macrophages under Low Exposure Doses".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 6.8(2018):10374-10384. |
入库方式： OAI收割
来源：生态环境研究中心
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