Organotin exposure stimulates steroidogenesis in H295R Cell via cAMP pathway
文献类型：期刊论文
| 作者 | Hu, Ligang; Yan, Xueting; He, Bin; Liu, Lihong; Qu, Guangbo; Shi, Jianbo; Liao, Chunyang; Jiang, Guibin
|
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2018-07-30 |
| 卷号 | 156页码:148-153 |
| 关键词 | Organotin compounds Steroid hormone Steroidogenesis H295R Cell Cyclic AMP |
| ISSN号 | 0147-6513 |
| 文献子类 | Article |
| 英文摘要 | Organotin compounds (OTs) are used in a range of industrial products, such as antifouling paints, agricultural pesticides and stabilizers. Owing to potential endocrine-disrupting effects, human exposure to such compounds is a concern. Nevertheless, little is known about the adverse effect of OTs on adrenocortical function in organisms. In this study, the human adrenocortical carcinoma cell (H295R) model was used to investigate effects of OTs on steroidogenesis and potential causes for such endocrine disruption was examined. H295R cells were exposed to several commonly used OTs, including triphenyltin (TPT), tributyltin (TBT), dibutyltin (DBT), and monobutyltin (MBT), and the production level of steroid hormones were quantified. TPT and TBT decreased the production levels of 17 beta-estradiol, aldosterone, and cortisol, but increased that of testosterone. Furthermore, the expression levels of ten major steroidogenic genes (HMGR, StAR, CYP11A1, 3 beta HSD2, CYP17, CYP19A1, CYP21, CYP11B1, CYP11B2, and 17 beta HSD) were examined and both up-regulation of CYP11B2 and down-regulation of StAR, 3 beta HSD2, CYP19A1, CYP21 and CYP11B1 by TPT and TBT were observed. Intracellular levels of ATP and cyclic adenosine monophosphate (cAMP) and the activity of adenylate cyclase (AC) decreased in the H295R cells treated with TPT and TBT. No obvious changes in H295R were found with the treatment of DBT and MBT. These results suggest that OTs may stimulate steroidogenesis in vitro via inhibition of cAMP signaling pathway. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41007]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Hu, Ligang,Yan, Xueting,He, Bin,et al. Organotin exposure stimulates steroidogenesis in H295R Cell via cAMP pathway[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,156:148-153. |
| APA | Hu, Ligang.,Yan, Xueting.,He, Bin.,Liu, Lihong.,Qu, Guangbo.,...&Jiang, Guibin.(2018).Organotin exposure stimulates steroidogenesis in H295R Cell via cAMP pathway.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,156,148-153. |
| MLA | Hu, Ligang,et al."Organotin exposure stimulates steroidogenesis in H295R Cell via cAMP pathway".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 156(2018):148-153. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。