Two-dimensional (weak anion exchange chromatography-gel electrophoresis) separations coupling to inductively coupled plasma mass spectrometry strategy for analysis of metalloproteins
文献类型：期刊论文
| 作者 | Yan, Xueting; He, Bin; Wang, Dingyi; Hu, Ligang; Liu, Lihong; Liao, Chunyang; Jiang, Guibin
|
| 刊名 | TALANTA
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 184页码:404-410 |
| 关键词 | Two-dimensional Metalloprotein ICP-MS Weak anion exchange chromatography Gel electrophoresis |
| ISSN号 | 0039-9140 |
| 文献子类 | Article |
| 英文摘要 | A two-dimensional (2-D, weak anion exchange chromatography-gel electrophoresis, WAX-GE) separations coupling to inductively coupled plasma mass spectrometry (ICP-MS) strategy was developed for efficient tracking of metalloproteins. Samples were fractionized with the primary dimension (WAX) and the collected fractions were subsequently subjected to the secondary dimension (GE) and finally detected with ICP-MS. The metalloproteins of interest from the secondary dimension were online split and collected for further manipulation. The molecular weight of metalloprotein was calibrated with a home-made protein marker including six iodine-labelled proteins with molecular weights ranging from 14 kDa to 77 kDa. The developed 2-D system is of higher separation resolution as compared to one-dimensional (1-D) system. Metalloproteins in Escherichia coli were further examined for validation of the method preformation. Several known or unknown metal-associated proteins were identified, evidencing the feasibility of the proposed method. Taken together, we established a solid methodology for efficient separation and qualitative study of metalloproteomics, providing a new strategy for the metallomics research. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41078]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yan, Xueting,He, Bin,Wang, Dingyi,et al. Two-dimensional (weak anion exchange chromatography-gel electrophoresis) separations coupling to inductively coupled plasma mass spectrometry strategy for analysis of metalloproteins[J]. TALANTA,2018,184:404-410. |
| APA | Yan, Xueting.,He, Bin.,Wang, Dingyi.,Hu, Ligang.,Liu, Lihong.,...&Jiang, Guibin.(2018).Two-dimensional (weak anion exchange chromatography-gel electrophoresis) separations coupling to inductively coupled plasma mass spectrometry strategy for analysis of metalloproteins.TALANTA,184,404-410. |
| MLA | Yan, Xueting,et al."Two-dimensional (weak anion exchange chromatography-gel electrophoresis) separations coupling to inductively coupled plasma mass spectrometry strategy for analysis of metalloproteins".TALANTA 184(2018):404-410. |
入库方式： OAI收割
来源：生态环境研究中心
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