Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method
文献类型：期刊论文
| 作者 | Li, Hong-Zhe; Yang, Xiao-Ru; Zhu, Yong-Guan; Penuelas, Josep; Ding, Kai; Zhou, Guo-Wei; Bi, Qing-Fang; Zhang, Ding-Peng; Ibrahim, Muhammad; Zheng, Bang-Xiao |
| 刊名 | AMB EXPRESS
 |
| 出版日期 | 2018-03-27 |
| 卷号 | 8页码:- |
| 关键词 | Phosphorus Inorganic phosphate solubilizing bacteria Isolation Characterization |
| ISSN号 | 2191-0855 |
| 文献子类 | Article |
| 英文摘要 | The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megaterium for releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P < 0.001), suggesting that organic acid may mobilize microbial P. Our study provides an efficient and effective method for identifying and analyzing the growth of iPSB strains able to solubilize inorganic P and gives a better understanding of the mechanism of P solubilization. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40570]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Hong-Zhe,Yang, Xiao-Ru,Zhu, Yong-Guan,et al. Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method[J]. AMB EXPRESS,2018,8:-. |
| APA | Li, Hong-Zhe.,Yang, Xiao-Ru.,Zhu, Yong-Guan.,Penuelas, Josep.,Ding, Kai.,...&Zheng, Bang-Xiao.(2018).Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method.AMB EXPRESS,8,-. |
| MLA | Li, Hong-Zhe,et al."Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method".AMB EXPRESS 8(2018):-. |
入库方式： OAI收割
来源：生态环境研究中心
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