Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment
文献类型：期刊论文
| 作者 | Ye, Mao; Sun, Mingming; Zhao, Yuanchao; Jiao, Wentao; Xia, Bing; Liu, Manqiang; Feng, Yanfang; Zhang, Zhongyun; Huang, Dan; Huang, Rong |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 241页码:978-987 |
| 关键词 | Polyvalent bacteriophage therapy Biochar Antibiotic resistance genes Escherichia coli K-12 Pseudomonas aeruginosa PAO1 |
| ISSN号 | 0269-7491 |
| 文献子类 | Article |
| 英文摘要 | High abundances of antibiotic-resistant pathogenic bacteria (ARPB) and antibiotic resistance genes (ARGs) in agricultural soil-plant systems have become serious threats to human health and environmental safety. Therefore, it is crucial to develop targeted technology to control existing antibiotic resistance (AR) contamination and potential dissemination in soil-plant systems. In this work, polyvalent bacteriophage (phage) therapy and biochar amendment were applied separately and in combination to stimulate ARPB/ARG dissipation in a soil-lettuce system. With combined application of biochar and polyvalent phage, the abundance of Escherichia coli K-12 (tet(R)) and Pseudomonas aeruginosa PAO1 (amp(R) + fos(R)) and their corresponding ARGs (tetM, tetQ, tetW, ampC, and fosA) significantly decreased in the soil after 63 days' incubation (p < 0.05). Similar results for endophytic K-12 and PAO1, and ARGs, were also obtained in lettuce tissues following combined treatment. Additionally, high throughput sequencing revealed that biochar and polyvalent phage synergetically improved the structural diversity and functional stability of the indigenous bacterial communities in soil and the endophytic ones in lettuce. Hence, this work proposes a novel biotechnology that combines biochar amendment and polyvalent phage therapy to achieve targeted inactivation of ARPB, which stimulates ARG dissipation in soil-lettuce systems. (C) 2018 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/40673]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Ye, Mao,Sun, Mingming,Zhao, Yuanchao,et al. Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment[J]. ENVIRONMENTAL POLLUTION,2018,241:978-987. |
| APA | Ye, Mao.,Sun, Mingming.,Zhao, Yuanchao.,Jiao, Wentao.,Xia, Bing.,...&Hu, Feng.(2018).Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment.ENVIRONMENTAL POLLUTION,241,978-987. |
| MLA | Ye, Mao,et al."Targeted inactivation of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa in a soil-lettuce system by combined polyvalent bacteriophage and biochar treatment".ENVIRONMENTAL POLLUTION 241(2018):978-987. |
入库方式： OAI收割
来源：生态环境研究中心
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