Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats
文献类型：期刊论文
| 作者 | Guo, Baoyuan; Zhang, Yanfeng; Xu, Peng; Li, Jianzhong; Wang, Huili ; Li, Wei; Li, Jitong; Chang, Jing; Zhu, Feilong; Hu, Xiao
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2018-08-07 |
| 卷号 | 52期号:15页码:8830-8837 |
| ISSN号 | 0013-936X |
| 文献子类 | Article |
| 英文摘要 | Myclobutanil (MT), a chiral fungicide, can be metabolized enantioselectively in organisms. In this work, the associated absorption, distribution, metabolism and transcriptional responses of MT in rats were determined following a single-dose (10 mg.kg(-1) body weight) exposure to rac-, (+)- or (-)-MT. The enantiomer fractions (EFs) were less than 0.5 with time in the liver, kidney, heart, lung, and testis, suggesting preferential enrichment of (-)-MT in these tissues. Furthermore, there was conversion of (+)-form to (-)-form in the liver and kidney after 6 h exposure to enantiopure (+)-MT. Enrichment and degradation of the two enantiomers differed between rac-MT and MT-enantiomers groups, suggesting that MT bioaccumulation is enantiomer-specific. Interestingly, the degradation half-life of MT in the liver with rac-MT treatment was shorter than that with both MT-enantiomer treatments. One reason may be that the gene expression levels of cytochrome P450 1a2 (cyp1a2) and cyp3a2 genes in livers treated with rac-MT were the highest among the three exposure groups. In addition, a positive correlation between the expression of cyp2e1 and cyp3a2 genes and rac-MT concentration was found in livers exposed to rac-MT. Simultaneously, five chiral metabolites were detected, and the enantiomers of three metabolites, RH-9090, RH-9089, and M2, were separated. The detected enantiomers of (+)-MT metabolites were in complete contrast with those of (-)-MT metabolites. According to the results, a metabolic pathway of MT in male rats was proposed, which included the following five metabolites: RH-9089, RH-9090, RH-9090 Sulfate, M1, and M2. The possible metabolic enzymes were marked in the pathway. The findings of this study provide more specific insights into the enantioselective metabolic mechanism of chiral triazole fungicides. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41252]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Baoyuan,Zhang, Yanfeng,Xu, Peng,et al. Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2018,52(15):8830-8837. |
| APA | Guo, Baoyuan.,Zhang, Yanfeng.,Xu, Peng.,Li, Jianzhong.,Wang, Huili.,...&Hao, Weiyu.(2018).Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats.ENVIRONMENTAL SCIENCE & TECHNOLOGY,52(15),8830-8837. |
| MLA | Guo, Baoyuan,et al."Enantioselective Distribution, Degradation, and Metabolite Formation of Myclobutanil and Transcriptional Responses of Metabolic-Related Genes in Rats".ENVIRONMENTAL SCIENCE & TECHNOLOGY 52.15(2018):8830-8837. |
入库方式： OAI收割
来源：生态环境研究中心
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