Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants
文献类型：期刊论文
| 作者 | Xu, Meiying; Deng, Ye; Yin, Huaqun; Chen, Hongrui; Zhang, Yu ; Li, Shuzhen; Feng, Kai; Wei, Ziyan
|
| 刊名 | ENVIRONMENT INTERNATIONAL
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 117页码:186-195 |
| 关键词 | 16S rRNA sul1 Degenerate primer design Quantitative PCR MiSeq sequencing |
| ISSN号 | 0160-4120 |
| 文献子类 | Article |
| 英文摘要 | An updated sul1 gene sequence database was constructed and new degenerate primers were designed to better investigate the abundance, diversity, and variation of a ubiquitous antibiotic resistance gene, sul1, with PCR-based methods in activated sludge from wastewater treatment plants (WWTPs). The newly designed degenerate primers showed high specificity and higher coverage in both in-silico evaluations and activated sludge samples compared to previous sul1 primers. Using the new primers, the abundance and diversity of sul1 gene, together with 16S rRNA gene, in activated sludge from five WWTPs in summer and winter were determined by quantitative PCR and MiSeq sequencing. The sul1 gene was found to be prevalent and displayed a comparable abundance (0.081 copies per bacterial cell in average) to the total bacteria across all samples. However, compared to the significant seasonal and geographical divergences in the quantity and diversity of bacterial communities in WWTPs, there were no significant seasonal or geographical variations of representative clusters of sul1 gene in most cases. Additionally, the representative sul1 clusters showed fairly close phylogeny and there was no obvious correlation between sul1 gene and the dominant bacterial genera, as well as the int1 gene, suggesting that bacterial hosts of sul1 gene is not stable, the sul1 gene may be carried by mobile genetic elements, sometimes integrated with class 1 integrons and sometimes not. Thus mobile genetic elements likely play a greater role than specific microbial taxa in determining the composition of sul1 gene in WWTPs. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41258]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu, Meiying,Deng, Ye,Yin, Huaqun,et al. Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants[J]. ENVIRONMENT INTERNATIONAL,2018,117:186-195. |
| APA | Xu, Meiying.,Deng, Ye.,Yin, Huaqun.,Chen, Hongrui.,Zhang, Yu.,...&Wei, Ziyan.(2018).Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants.ENVIRONMENT INTERNATIONAL,117,186-195. |
| MLA | Xu, Meiying,et al."Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants".ENVIRONMENT INTERNATIONAL 117(2018):186-195. |
入库方式： OAI收割
来源：生态环境研究中心
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