Optimization of the medium for the growth of Nitrobacter winogradskyi by statistical method
文献类型：期刊论文
| 作者 | Zhuang, X.; Zhang, Y. ; Zhang, Y.; Gao, J.; Shen, Q.; Bai, Z.; Zhuang, G.
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| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 67期号:3页码:306-313 |
| 关键词 | carboxysomes central composite design culture conditions optimization Nitrobacter winogradskyi polyphosphates ultrastructure |
| ISSN号 | 0266-8254 |
| 文献子类 | Article |
| 英文摘要 | Although Nitrobacter winogradskyi is an important chemoorganotrophic organism for the study of nitrite-oxidizing bacteria physiology as well as nitrification, until now, the mixotrophic medium for this organism growth has not been optimized, comprehensively. In this study, we aimed to improve the growth medium of N.winogradskyi using the one-factor-at-a-time (NaNO2, glycerol, pH) method. In addition, a further experimental design was carried out based on central composite design with response surface methodology. Different combinations of the three cultural parameters were fitted by multiple regression analysis to calculate the predicted response. Our results suggest that optimal culture condition for the growth of N.winogradskyi was a modified DSMZ 756a medium containing NaNO2 (574gl(-1)) and glycerol (3788mmoll(-1)), pH 783, a temperature of 28 degrees C and agitation at 120revmin(-1). The results from a validation experiment (bacterial growth: OD600 10293) were close to the value predicted by the quadratic model (OD600 10994). In addition, we uncovered the potential mechanism at the cellular and ultrastructural levels. The results indicated that glycerol in themedia enhanced the rate of cell division and cell growth by increasing the accumulation of polyphosphates and phosphorus, and high concentrations of NaNO2 provided sufficient energy for growth and contributed to the generation of carboxysomes in cells for CO2 fixation. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41284]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhuang, X.,Zhang, Y.,Zhang, Y.,et al. Optimization of the medium for the growth of Nitrobacter winogradskyi by statistical method[J]. LETTERS IN APPLIED MICROBIOLOGY,2018,67(3):306-313. |
| APA | Zhuang, X..,Zhang, Y..,Zhang, Y..,Gao, J..,Shen, Q..,...&Zhuang, G..(2018).Optimization of the medium for the growth of Nitrobacter winogradskyi by statistical method.LETTERS IN APPLIED MICROBIOLOGY,67(3),306-313. |
| MLA | Zhuang, X.,et al."Optimization of the medium for the growth of Nitrobacter winogradskyi by statistical method".LETTERS IN APPLIED MICROBIOLOGY 67.3(2018):306-313. |
入库方式： OAI收割
来源：生态环境研究中心
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