QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling
文献类型：期刊论文
| 作者 | Zheng, Bangxiao; Su, Jianqiang; Penuelas, Josep; Sardans, Jordi; Zhu, Yongguan |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2018-01-12 |
| 卷号 | 61期号:12页码:1451-1462 |
| 关键词 | microbial genes functional potential high-throughput qPCR elemental cycling biogeochemical cycle ecological process |
| ISSN号 | 1674-7305 |
| 文献子类 | Article |
| 英文摘要 | Microorganisms are major drivers of elemental cycling in the biosphere. Determining the abundance of microbial functional traits involved in the transformation of nutrients, including carbon (C), nitrogen (N), phosphorus (P) and sulfur (S), is critical for assessing microbial functionality in elemental cycling. We developed a high-throughput quantitative-PCR-based chip, Quantitative microbial element cycling (QMEC), for assessing and quantifying the genetic potential of microbiota to mineralize soil organic matter and to release C, N, P and S. QMEC contains 72 primer pairs targeting 64 microbial functional genes for C, N, P, S and methane metabolism. These primer pairs were characterized by high coverage (average of 18-20 phyla covered per gene) and sufficient specificity (>70% match rate) with a relatively low detection limit (7-102 copies per run). QMEC was successfully applied to soil and sediment samples, identifying significantly different structures, abundances and diversities of the functional genes (P<0.05). QMEC was also able to determine absolute gene abundance. QMEC enabled the simultaneous qualitative and quantitative determination of 72 genes from 72 samples in one run, which is promising for comprehensively investigating microbially mediated ecological processes and biogeochemical cycles in various environmental contexts including those of the current global change. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41226]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Zheng, Bangxiao,Su, Jianqiang,Penuelas, Josep,et al. QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling[J]. SCIENCE CHINA-LIFE SCIENCES,2018,61(12):1451-1462. |
| APA | Zheng, Bangxiao,Su, Jianqiang,Penuelas, Josep,Sardans, Jordi,&Zhu, Yongguan.(2018).QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling.SCIENCE CHINA-LIFE SCIENCES,61(12),1451-1462. |
| MLA | Zheng, Bangxiao,et al."QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling".SCIENCE CHINA-LIFE SCIENCES 61.12(2018):1451-1462. |
入库方式： OAI收割
来源：生态环境研究中心
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