New cytokines and TLR pathway signaling molecules in Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression, and their response to chlorpyrifos
文献类型：期刊论文
| 作者 | Qin, Jianhui; Zha, Jinmiao; Yuan, Lilai; Chen, Rui; Hong, Xiangsheng; Zhao, Xu |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 199页码:26-34 |
| 关键词 | Chinese rare minnow Cytokines TLR pathway signaling molecule Chlorpyrifos Clone |
| ISSN号 | 0045-6535 |
| 文献子类 | Article |
| 英文摘要 | In this study, the cDNA fragments of cytokines (i1-8) and toll-like receptor (TLR) pathway signaling molecules (myd88, irak-1, irf5, and irj7) in the Chinese rare minnow were cloned and exhibited a high amino-acid sequence identity compared to other cyprinid fish orthologs. The mRNA expressions of these genes in the different tissues (liver, brain, spleen, kidney, and skin) were observed. The highest expression levels of myd88, irak-1, and irf5 were detected in the spleen, whereas iI-8 and irf7 were detected in the kidney and liver respectively. The mRNA expression of irak-1, irf5, and irf7 in the liver from 0.1 mu g/L. and 0.5 mu g/L CPF treatments were significantly increased on day 7 (p < 0.05), whereas the levels of only irak-1 and irf7 were markedly increased on day 28 (p < 0.05). Moreover, the mRNA expression of il-8 in the spleen following 0.5 mu g/L CPF treatments was significantly decreased on day 7 (p < 0.05), whereas significantly decrease were observed in the levels of irf7 in the spleen at 2.5 mu g/L CPF on days 7 and 28 (p < 0.05). The 0.1 mu g/t. and 0.5 mu g/L of CPF significantly induced the levels of irak-1 and myd88 in the spleen after 28 d exposure (p < 0.05). Therefore, the high induction of cytokines and TLR pathway signaling molecules demonstrated that Chinese rare minnow was immune-compromised exposed to CPF. Moreover, our finding indicated that these immune-related genes could be feasible to screen for substances hazardous to the immune system of fish. (C) 2018 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/41389]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Qin, Jianhui,Zha, Jinmiao,Yuan, Lilai,et al. New cytokines and TLR pathway signaling molecules in Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression, and their response to chlorpyrifos[J]. CHEMOSPHERE,2018,199:26-34. |
| APA | Qin, Jianhui,Zha, Jinmiao,Yuan, Lilai,Chen, Rui,Hong, Xiangsheng,&Zhao, Xu.(2018).New cytokines and TLR pathway signaling molecules in Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression, and their response to chlorpyrifos.CHEMOSPHERE,199,26-34. |
| MLA | Qin, Jianhui,et al."New cytokines and TLR pathway signaling molecules in Chinese rare minnow (Gobiocypris rarus): Molecular characterization, basal expression, and their response to chlorpyrifos".CHEMOSPHERE 199(2018):26-34. |
入库方式： OAI收割
来源：生态环境研究中心
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